CAJ | 학술논문

建立了免疫亲和柱净化-光化学衍生高效液相色谱荧光法测定植物油中黄曲霉毒素B1含量的方法，并对样品的测定条件进行了优化。结果表明：植物油的称样量缩减为5．00 g，黄曲霉毒素B1测定结果与GB/T 18979-2003测定结果基本吻合；工作曲线在1~40 ng/mL质量浓度范围内具有良好的线性关系，相关系数为0．9999，方法检出限为0．3μg/kg；在空白样品中添加低、中、高3个不同添加量水平的黄曲霉毒素B1标准品，其回收率在90．5%~99．8%之间，相对标准偏差在6．3%~9．8%之间。采用光化学衍生，操作简单，无需衍生剂，避免了柱前衍生和柱后衍生受衍生剂浓度、温度、反应时间的影响，所建立的方法适用于植物油中黄曲霉毒素B1含量的测定。
건립료면역친화주정화-광화학연생고효액상색보형광법측정식물유중황곡매독소B1함량적방법，병대양품적측정조건진행료우화。결과표명：식물유적칭양량축감위5．00 g，황곡매독소B1측정결과여GB/T 18979-2003측정결과기본문합；공작곡선재1~40 ng/mL질량농도범위내구유량호적선성관계，상관계수위0．9999，방법검출한위0．3μg/kg；재공백양품중첨가저、중、고3개불동첨가량수평적황곡매독소B1표준품，기회수솔재90．5%~99．8%지간，상대표준편차재6．3%~9．8%지간。채용광화학연생，조작간단，무수연생제，피면료주전연생화주후연생수연생제농도、온도、반응시간적영향，소건립적방법괄용우식물유중황곡매독소B1함량적측정。
Aflatoxin B1 in vegetable oil was determined by HPLC-FLD with photochemical derivatization after immunoaffinity column purification, and the determination conditions of sample were optimized. The results showed that the aflatoxin B1 in vegetable oil determined by the established method coincided with the results determined by the national standard method GB/T 18979-2003 when the mass of sample was reduced to 5. 00 g. The standard curve had a good linearity when the aflatoxin B1 content was in the range of 1- 40 ng/mL. The correlation coefficient, the limit of detection, the recovery rate and the relative standard deviation of the method were 0. 999 9,0. 3 μg/kg,90. 5% -99. 8% and 6. 3% -9. 8% respec-tively. The method had items of simple operation, no derivatization agent,avoidance of the influences of derivatization agent concentration, temperature and reaction time in the processes of pre -column and post - column derivatizations, which was applicable to determine the content of aflatoxin B1 in vegetable oil.