CAJ | 학술논문

研究了布那迪栅藻( Scenedesmus bernardii)异养培养条件及不同碳源、氮源对其生化组成的影响。结果表明：异养培养时所需的最佳碳源为葡萄糖，其最适质量浓度为50．0 g/L；最佳氮源为酵母提取物，其最适质量浓度为2．7 g/L；摇瓶培养时所获得的最大生物量达24．8 g/L。布那迪栅藻异养培养时细胞内主要积累碳水化合物，其含量达到细胞干重的58．2%，最高产率为1．05 g/(L·d)；其次积累总脂，含量为细胞干重的30．9%，最高产率为0．64 g/(L·d)。
연구료포나적책조( Scenedesmus bernardii)이양배양조건급불동탄원、담원대기생화조성적영향。결과표명：이양배양시소수적최가탄원위포도당，기최괄질량농도위50．0 g/L；최가담원위효모제취물，기최괄질량농도위2．7 g/L；요병배양시소획득적최대생물량체24．8 g/L。포나적책조이양배양시세포내주요적루탄수화합물，기함량체도세포간중적58．2%，최고산솔위1．05 g/(L·d)；기차적루총지，함량위세포간중적30．9%，최고산솔위0．64 g/(L·d)。
The heterotrophic cultivation conditions of Scenedesmus bernardii and the different carbon sources and nitrogen sources on its biochemical components were studied. The results showed that the best carbon source for Scenedesmus bernardii under heterotrophic growth was glucose, and its optimal mass concentration was 50 . 0 g/L; the best nitrogen source for Scenedesmus bernardii under heterotrophic growth was yeast extract, and its optimal mass concentration was 2. 7 g/L. Under the optimal heterotroph-ic growth conditions, the maximum biomass was up to 24. 8 g/L. The carbohydrates mainly accumulated in the cell of Scenedesmus bernardii under heterotrophic growth conditions, accounting for 58. 2% of the cell dry weight, and the highest yield was 1. 05 g/( L·d); the next was the total lipid, accounting for 30. 9% of the cell dry weight, and the highest yield was 0. 64 g/(L·d).