中国油脂
中國油脂
중국유지
CHINA OILS AND FATS
2014年
6期
12-17
,共6页
彭瑶瑶%王千千%王爱梅%王洪新
彭瑤瑤%王韆韆%王愛梅%王洪新
팽요요%왕천천%왕애매%왕홍신
水酶法%牡丹籽油%GC-MS
水酶法%牡丹籽油%GC-MS
수매법%모단자유%GC-MS
aqueous enzymatic method%Paeonia suf-fruticosa seed oil%GC-MS
采用水酶法从牡丹籽中提取牡丹籽油，通过对比实验，选择了三步酶解结合二次破乳的工艺流程。5 g牡丹籽粉三步酶解结合二次破乳提取牡丹籽油的优化条件为：料水比1：5，细胞壁多糖水解酶(纤维素酶与果胶酶配比2：1)加酶量1．5 mL，酶解时间2．5 h；α-中温淀粉酶加酶量0．6 mL，酶解时间45 min；碱性蛋白酶加酶量0．18 g，酶解时间2 h；冷冻解冻破乳法，-20℃冷冻18 h后50℃解冻2 h。在优化条件下取200 g牡丹籽粉提取牡丹籽油，其游离油得率达到17．6%，总油得率达到25．4%，所得牡丹籽油品质良好，未检出过氧化物，酸值(KOH)3．5 mg/g，碘值(I)177．09 g/100 g，皂化值(KOH)173．07 mg/g；牡丹籽油中不饱和脂肪酸含量达到92．77%，其中亚麻酸含量37．33%，亚油酸含量31．13%，油酸含量24．31%。水酶法提取牡丹籽油具有牡丹籽粉无需干燥，整个提油过程温度不超过70℃的优点，可大大减少提取过程中油脂的氧化。
採用水酶法從牡丹籽中提取牡丹籽油，通過對比實驗，選擇瞭三步酶解結閤二次破乳的工藝流程。5 g牡丹籽粉三步酶解結閤二次破乳提取牡丹籽油的優化條件為：料水比1：5，細胞壁多糖水解酶(纖維素酶與果膠酶配比2：1)加酶量1．5 mL，酶解時間2．5 h；α-中溫澱粉酶加酶量0．6 mL，酶解時間45 min；堿性蛋白酶加酶量0．18 g，酶解時間2 h；冷凍解凍破乳法，-20℃冷凍18 h後50℃解凍2 h。在優化條件下取200 g牡丹籽粉提取牡丹籽油，其遊離油得率達到17．6%，總油得率達到25．4%，所得牡丹籽油品質良好，未檢齣過氧化物，痠值(KOH)3．5 mg/g，碘值(I)177．09 g/100 g，皂化值(KOH)173．07 mg/g；牡丹籽油中不飽和脂肪痠含量達到92．77%，其中亞痳痠含量37．33%，亞油痠含量31．13%，油痠含量24．31%。水酶法提取牡丹籽油具有牡丹籽粉無需榦燥，整箇提油過程溫度不超過70℃的優點，可大大減少提取過程中油脂的氧化。
채용수매법종모단자중제취모단자유，통과대비실험，선택료삼보매해결합이차파유적공예류정。5 g모단자분삼보매해결합이차파유제취모단자유적우화조건위：료수비1：5，세포벽다당수해매(섬유소매여과효매배비2：1)가매량1．5 mL，매해시간2．5 h；α-중온정분매가매량0．6 mL，매해시간45 min；감성단백매가매량0．18 g，매해시간2 h；냉동해동파유법，-20℃냉동18 h후50℃해동2 h。재우화조건하취200 g모단자분제취모단자유，기유리유득솔체도17．6%，총유득솔체도25．4%，소득모단자유품질량호，미검출과양화물，산치(KOH)3．5 mg/g，전치(I)177．09 g/100 g，조화치(KOH)173．07 mg/g；모단자유중불포화지방산함량체도92．77%，기중아마산함량37．33%，아유산함량31．13%，유산함량24．31%。수매법제취모단자유구유모단자분무수간조，정개제유과정온도불초과70℃적우점，가대대감소제취과정중유지적양화。
The Paeonia suffruticosa see d oil was extracted by aqueous enzymatic method. According to comparative experiments,the three-step enzymolysis combined with twice demulsification was chose as the optimal process. The results showed that the extraction conditions of Paeonia suffruticosa seed oil by three-step enzymolysis combined with twice demulsification were optimized as follows:5 g Paeonia suf-fruticosa seed powder, ratio of material to water 1:5, dosage of cell wall polysaccharides hydrolysis en-zyme (ratio of Cellelase to Pectinase 2:1) 1. 5 mL,enzymolysis time 2. 5 h;α-amylase dosage 0. 6 mL, enzymolysis time 45 min;alkaline protease dosage 0. 18 g,enzymolysis time 2 h;using freezing and tha-wing demulsification method,freezing temperature -20℃,freezing time 18 h,thawing temperature 50℃, thawing time 2 h. When extracting oil from 200 g of Paeonia suffruticosa seed powder under the optimal conditions,the free oil yield was 17. 6% and the total oil yield was 25. 4%. The Paeonia suffruticosa seed oil obtained had good quality in terms of undetected peroxide,the acid value 3. 5 mgKOH/g,iodine value 177. 09 gI/100 g and saponification value 173. 07 mgKOH/g. The unsaturated fatty acids content of Pae-onia suffruticosa seed oil was up to 92. 77%,in which the contents of linolenic acid,linoleic acid and ole-ic acid were 37. 33%,31. 13% and 24. 31% respectively. The aqueous enzymatic extraction of Paeonia suffruticosa seed oil had the items of the seed powder without drying and the extraction temperature below 70℃ during the whole process , which could reduce the chances of oil oxidation.
