CAJ | 학술논문

目的：原花青素的致突变性和抗氧化性研究。方法用Ames试验、小鼠微核试验和小鼠精子畸形试验研究原花青素的致突变性。按老龄小鼠血中丙二醛（ MDA）值分为5组（每组10只），设空白对照组、溶剂对照组、41.67、83.33及250.0 mg/kg体重3个剂量组，观察给药后鼠血中超氧化物歧化酶（ SOD）、谷胱甘肽过氧化物酶（ GSH-Px）、丙二醛（ MDA）水平的变化。结果 Ames试验、小鼠骨髓细胞微核试验和小鼠精子畸形试验结果均为阴性。与空白对照组和溶剂对照组比较，83.33、250.0 mg/kg组老龄小鼠实验后红细胞MDA含量明显降低，250.0 mg/kg组全血GSH-PX活力明显增高（ P<0.05）。结论在本实验条件下，原花青素未显示有致突变性；原花青素对老龄小鼠具有抗氧化功能。
목적：원화청소적치돌변성화항양화성연구。방법용Ames시험、소서미핵시험화소서정자기형시험연구원화청소적치돌변성。안노령소서혈중병이철（ MDA）치분위5조（매조10지），설공백대조조、용제대조조、41.67、83.33급250.0 mg/kg체중3개제량조，관찰급약후서혈중초양화물기화매（ SOD）、곡광감태과양화물매（ GSH-Px）、병이철（ MDA）수평적변화。결과 Ames시험、소서골수세포미핵시험화소서정자기형시험결과균위음성。여공백대조조화용제대조조비교，83.33、250.0 mg/kg조노령소서실험후홍세포MDA함량명현강저，250.0 mg/kg조전혈GSH-PX활력명현증고（ P<0.05）。결론재본실험조건하，원화청소미현시유치돌변성；원화청소대노령소서구유항양화공능。
Objective To examine the mutagenicity and antioxidant activity of Proanthocyanidin( PC). Methods Three mutagenicity tests including Ames test,bone marrow micronucleus test and sperm malformation test in mice were conducted. According to the level of erythrocyte MDA,11-month-old rats were randomly divided into five groups including blank control,solvent control and 41. 67,83. 33,250. 0 mg/kg test groups. The content of erythrocyte MDA,SOD and GSH-PX activity of each dosage group were then observed after administration of PC to test the antioxidant activity. Results In all three mutagenicity tests,no mutagenic effect was observed in any PC - treated group. Compared with two control groups,the content of erythrocyte MDA was significantly decreased in 83. 33 and 250. 0 mg/kg groups(both P<0. 05) and GSH-Px activity was significantly increased in 250 mg/kg group(P<0. 05). Conclusion PC had no mutagenic effect but showed antioxidant activity under our experimental conditions.