CAJ | 학술논문

目的：探讨核苷酸切除修复交叉互补基因1（ ERCC1），乳腺癌易感基因1（ BRCA1）的表达与原发性上皮性卵巢癌患者临床病理特征及铂类化疗敏感性的关系，以评价ERCC1和BRCA1基因对原发性上皮性卵巢癌铂类化疗敏感性的预测价值。方法收集48例接受卵巢癌分期手术及满意或较满意的肿瘤细胞减灭术的原发性上皮性卵巢癌患者肿瘤标本，采用免疫组化方法检测ERCC1及BRCA1的表达，并分析 ERCC1及BRCA1基因表达与卵巢上皮性癌临床病理特征、临床化疗疗效的关系。结果 ERCC1的表达与原发性卵巢上皮性癌患者的临床分期有关（ P﹤0.05），与年龄、病理类型、分化程度均无关（ P﹥0.05），BRCA1的表达与原发性卵巢上皮性癌患者年龄、病理类型、分化程度及临床分期均无关（ P﹥0.05）；耐药者ERCC1高表达率高于敏感者，差异有统计学意义（ P﹤0.05），耐药者BRCA1高表达率高于敏感者，但差异无统计学意义（ P﹥0.05）；ERCC1在卵巢上皮性癌中阳性表达率为89.58％，BRCA1在卵巢上皮性癌中阳性表达率为25.00％。结论 ERCC1的表达与原发性卵巢上皮性癌患者的临床分期有关，与年龄、病理类型、分化程度均无关，BRCA1的表达与原发性卵巢上皮癌患者的临床病理特征无关；ERCC1在卵巢上皮性癌中的阳性表达率高，与临床含铂类化疗方案的敏感性有关；BRCA1在卵巢上皮性癌中的阳性表达率低。
목적：탐토핵감산절제수복교차호보기인1（ ERCC1），유선암역감기인1（ BRCA1）적표체여원발성상피성란소암환자림상병리특정급박류화료민감성적관계，이평개ERCC1화BRCA1기인대원발성상피성란소암박류화료민감성적예측개치。방법수집48례접수란소암분기수술급만의혹교만의적종류세포감멸술적원발성상피성란소암환자종류표본，채용면역조화방법검측ERCC1급BRCA1적표체，병분석 ERCC1급BRCA1기인표체여란소상피성암림상병리특정、림상화료료효적관계。결과 ERCC1적표체여원발성란소상피성암환자적림상분기유관（ P﹤0.05），여년령、병리류형、분화정도균무관（ P﹥0.05），BRCA1적표체여원발성란소상피성암환자년령、병리류형、분화정도급림상분기균무관（ P﹥0.05）；내약자ERCC1고표체솔고우민감자，차이유통계학의의（ P﹤0.05），내약자BRCA1고표체솔고우민감자，단차이무통계학의의（ P﹥0.05）；ERCC1재란소상피성암중양성표체솔위89.58％，BRCA1재란소상피성암중양성표체솔위25.00％。결론 ERCC1적표체여원발성란소상피성암환자적림상분기유관，여년령、병리류형、분화정도균무관，BRCA1적표체여원발성란소상피암환자적림상병리특정무관；ERCC1재란소상피성암중적양성표체솔고，여림상함박류화료방안적민감성유관；BRCA1재란소상피성암중적양성표체솔저。
Objective To investigate the association of ERCC1 and BRCA1 gene expression with clinical features and sensitivity to platinum-containing chemotherapy in patients with ovarian epithelial carcinoma. Methods Primary ovarian epithelial carcinoma tissues were harvested from 48 patients receiving staging surgery or cytoreductive surgery. Expression of ERCC1 and BRCA1 in the tumor samples was detected by immunohistochemistry. Results ERCC1 expression was correlated with clinical stage(P﹤0. 05)but not with age,pathological type or degree of differentiation(P﹥0. 05). BRCA1 expression was not correlated with any of the clinicopathological features(P﹥0. 05);ERCC1 expression was significantly higher in drug-resistant tissues than in drug-sensitive samples(P﹤0. 05);The expression of ERCC1 and BRCA1 was positive in 89. 58%and 25. 00%of the samples,respectively. Conclusion ERCC1 gene expression is correlated with clinical stage but not with age,pathological type or degree of differentiation. BRCA1 gene expression is not correlated with clinicopathological features. ERCC1 has high positive expression in epithelial ovarian cancer and is correlated with sensitivity of platinum-containing adjuvant chemotherapy.