CAJ | 학술논문

目的：观察黄丹胶囊对寒冷刺激小鼠模型的唾液中分泌型免疫球蛋白A（ SIgA）含量和溶菌酶活性的影响。方法将小鼠随机分为5组：对照组、模型组、黄丹胶囊高剂量治疗组、中剂量治疗组、低剂量治疗组。除对照组外，所有动物置于-20℃寒冷环境中刺激15 min，1次/d，连续3 d；黄丹胶囊高剂量、中等剂量、低剂量组3组小鼠于寒冷刺激前1 h，各组分别按1、2、4 g/kg剂量灌胃给予黄丹胶囊。第3 d寒冷刺激后，每只小鼠腹腔注射0.1％毛果芸香碱注射液后取小鼠唾液，测定唾液中SIgA含量和溶菌酶活性。结果经寒冷刺激后，小鼠唾液中SIgA水平与溶菌酶活性均显著下降；黄丹胶囊高剂量组SIgA含量较模型组非常显著地升高（P﹤0.01），中、小剂量组均有所上升但与模型组相比无显著差异（P﹥0.05），与高剂量组比较亦无显著差异（P﹥0.05）；黄丹胶囊高、中剂量组溶菌酶活性较模型组显著升高（P﹤0.05或P﹤0.01），中、高剂量组相比无显著差异，小剂量组活性有所增加，但与模型组相比无显著差异（ P﹥0.05），与中、高剂量组相比亦无显著差异（ P﹥0.05）。结论在寒冷刺激下，小鼠上呼吸道体液免疫受到抑制，而黄丹胶囊能够提高寒冷刺激小鼠模型上呼吸道黏膜体液免疫功能。
목적：관찰황단효낭대한랭자격소서모형적타액중분비형면역구단백A（ SIgA）함량화용균매활성적영향。방법장소서수궤분위5조：대조조、모형조、황단효낭고제량치료조、중제량치료조、저제량치료조。제대조조외，소유동물치우-20℃한랭배경중자격15 min，1차/d，련속3 d；황단효낭고제량、중등제량、저제량조3조소서우한랭자격전1 h，각조분별안1、2、4 g/kg제량관위급여황단효낭。제3 d한랭자격후，매지소서복강주사0.1％모과예향감주사액후취소서타액，측정타액중SIgA함량화용균매활성。결과경한랭자격후，소서타액중SIgA수평여용균매활성균현저하강；황단효낭고제량조SIgA함량교모형조비상현저지승고（P﹤0.01），중、소제량조균유소상승단여모형조상비무현저차이（P﹥0.05），여고제량조비교역무현저차이（P﹥0.05）；황단효낭고、중제량조용균매활성교모형조현저승고（P﹤0.05혹P﹤0.01），중、고제량조상비무현저차이，소제량조활성유소증가，단여모형조상비무현저차이（ P﹥0.05），여중、고제량조상비역무현저차이（ P﹥0.05）。결론재한랭자격하，소서상호흡도체액면역수도억제，이황단효낭능구제고한랭자격소서모형상호흡도점막체액면역공능。
Objective To observe the effect of Huangdan capsules on the secretion of secretory immunoglobulin A( SIgA)and lysozyme activity in saliva of the cold stimulated mouse model. Methods Mice were randomly divided into 5 groups:one control group, one cold stimulated model group and three treatment groups( Huangdan capsule at high dose,moderate dose and low dose, respectively);all animals,except those in control group,were stimulated at -20 ℃ cold environment for 15 minutes,once a day,for 3 consecutive days;Huangdan capsules at dose of 1,2,4 g/kg were given to mice in the three treatment groups,respectively by gavage 1 hour before cold stimulation every day;on the 3rd day,after cold stimulation,each mouse was given 0. 1% pilocarpine by celiac injection,and then saliva were collected,and the level of SIgA and the activity of lysozyme were detected. Results After cold stimulation for 3 days,the levels of SIgA and activity of lysozyme in the saliva of the mice decreased significantly,the level of SIgA of mice in high dose group were significantly higher than that of mice in model group(P﹤0. 01);the levels of SIgA of mice in moderate dose group and low dose group increased but were not significantly different from that of mice in model group(P﹥0. 05)and from that of mice in high dose group as well(P﹥0. 05);the activity of lysozyme of mice in high dose group and moderate dose group increased with no obvious difference in between but were much higher than that of mice in model group(P﹤0. 05 or P﹤0. 01),and the activity of the mice in low dose group had certain increase but no difference from that of mice in model group(P﹥0. 05),while no difference from those of mice in high dose group and moderate group(P﹥0. 05). Conclusions Huangdan capsule can improve humoral immune function of the upper respiratory tract mucous membrane in cold stimulated mouse model.