CAJ | 학술논문

目的：通过对上海地区333名健康成年人外周血T淋巴细胞亚群计数，调查正常人群T淋巴细胞亚群的参考区间，建立95％可信区间的参考区间，并分析和探讨其变化特点，为机体免疫状态的分析和临床诊疗提供参考。方法采用多参数三色免疫荧光标记外周血T淋巴细胞，使用BD FACSCalibur流式细胞仪检测并分析，获得T淋巴细胞亚群的百分比和绝对值。结果女性的CD3＋百分比、CD4＋/CD8＋比值、CD3＋CD4＋百分比和CD4＋CD45RA＋百分比分别为（72．6％±8．1％）、1．57（0．74～4．70）、（44．8％±8．0％）和（1．71％±6．3％），均高于男性分别为（70．9％±8．5％）、1．43（0．51～4．12）、（40．6％±9．3％）和（15．1％±6．8％）。男性的淋巴细胞绝对值计数、CD8＋CD28＋的绝对值计数分别为（2069．8±474．1）个/μL、（237．5±95．7）个/μL，均高于女性（1932．8±469．7）个/μL、（218．1±111．1）个/μL。T淋巴细胞亚群的百分比在不同年龄组之间随年龄增大而发生变化，年龄相差越大，差异越大，其中＞50岁组与其他组别比较差异有统计学意义。333名健康成年人外周血淋巴细胞绝对值计数为（1069．1～2935．6）个/μL，T淋巴细胞亚群百分比计数为：CD3＋细胞55．3％～87．6％， CD3＋CD4＋细胞25．2％～57．6％，CD3＋CD8＋细胞15．0％～42．1％，CD4＋CD25＋细胞2．5％～10．5％，CD4＋CD45RA＋细胞5．0％～27．2％，CD8＋CD28＋细胞4％～19．3％，CD4＋/CD8＋比值0．7～2．9。结论健康成年人T淋巴细胞亚群的百分比分布存在性别和年龄差异，由于这种差异不大，可以合并数据建立上海地区健康成年人外周血T淋巴细胞亚群的参考区间。
목적：통과대상해지구333명건강성년인외주혈T림파세포아군계수，조사정상인군T림파세포아군적삼고구간，건립95％가신구간적삼고구간，병분석화탐토기변화특점，위궤체면역상태적분석화림상진료제공삼고。방법채용다삼수삼색면역형광표기외주혈T림파세포，사용BD FACSCalibur류식세포의검측병분석，획득T림파세포아군적백분비화절대치。결과녀성적CD3＋백분비、CD4＋/CD8＋비치、CD3＋CD4＋백분비화CD4＋CD45RA＋백분비분별위（72．6％±8．1％）、1．57（0．74～4．70）、（44．8％±8．0％）화（1．71％±6．3％），균고우남성분별위（70．9％±8．5％）、1．43（0．51～4．12）、（40．6％±9．3％）화（15．1％±6．8％）。남성적림파세포절대치계수、CD8＋CD28＋적절대치계수분별위（2069．8±474．1）개/μL、（237．5±95．7）개/μL，균고우녀성（1932．8±469．7）개/μL、（218．1±111．1）개/μL。T림파세포아군적백분비재불동년령조지간수년령증대이발생변화，년령상차월대，차이월대，기중＞50세조여기타조별비교차이유통계학의의。333명건강성년인외주혈림파세포절대치계수위（1069．1～2935．6）개/μL，T림파세포아군백분비계수위：CD3＋세포55．3％～87．6％， CD3＋CD4＋세포25．2％～57．6％，CD3＋CD8＋세포15．0％～42．1％，CD4＋CD25＋세포2．5％～10．5％，CD4＋CD45RA＋세포5．0％～27．2％，CD8＋CD28＋세포4％～19．3％，CD4＋/CD8＋비치0．7～2．9。결론건강성년인T림파세포아군적백분비분포존재성별화년령차이，유우저충차이불대，가이합병수거건립상해지구건강성년인외주혈T림파세포아군적삼고구간。
Objective To establish the reference ranges of peripheral blood T lymphocyte subsets among 333 healthy adults in Shanghai,to analyze and discuss the change characteristics through the establishment of 95%confidence interval,and to provide the reference for immune status analysis and clinical diagnosis and treatment. Methods Multiparameter three-color immunofluorescence was used to stain the peripheral blood T lymphocytes,and BD FACSCalibur flow cytometry was used to acquire and analyze the percentages and absolute counts of T lymphocyte subsets.Results Among females,the CD3 +percentage,CD4 +/CD8 +ratio,CD3 +CD4 +percentage and CD4 +CD45RA+percentage (72.6% ±8.1%),1 .57(0.74 -4.70),(44.8% ±8.0%)and (1 .71% ±6.3%)were respectively higher than those among males (70.9%±8.5%),1 .43(0.51 -4.1 2),(40.6%±9.3%)and (1 5.1%±6.8%).The lymphocyte absolute count and CD8 +CD28 +absolute count in males (2 069.8 ±474.1 )cells/μL and (237.5 ±95.7) cells/μL were higher than those in females (1 932.8 ±469.7 ) cells/μL and (21 8.1 ± 1 1 1 .1 )cells/μL.The percentage of T lymphocyte subsets increased with the increase of age.There were statistical significances in >50 year-old group with the other groups.The absolute count of 333 healthy adults for peripheral blood T lymphocyte was (1 069.1 -2 935.6)cells/μL.The percentages of T lymphocyte subsets were:CD3 +55.3% -87.6%,CD3 +CD4 +25.2% -57.6%,CD3 +CD8 +1 5.0% -42.1%,CD4 +CD25 +2.5% -1 0.5%,CD4 +CD45RA+5.0%-27.2 % and CD8 +CD28 +4%-1 9.3%,and CD4 +/CD8 +ratio was 0.7 -2.9.Conclusions There are differences for the percentage distribution of T lymphocyte subsets among healthy adults between sex and age. Since these differences can be ignored,the reference ranges of peripheral blood T lymphocyte subsets among healthy adults could be established in Shanghai.