CAJ | 학술논문

根据动物种间特异性的原则，筛选出1对微卫星引物，建立了一种鉴别羊肉真伪的PCR检测技术。应用该方法，检测不同品种的羊肉样本（山羊、绵羊、杂交羊等）均能特异扩增出220～247 bp的目的片段，而猪、牛、鸡、鸭、兔等动物样本均呈阴性。该方法对羊肉的检测敏感度达10-10。应用该方法对山东省内屠宰场生产线、超市、市场中的各种畜禽鲜肉样品进行鉴定，羊肉的检出率为100％。
근거동물충간특이성적원칙，사선출1대미위성인물，건립료일충감별양육진위적PCR검측기술。응용해방법，검측불동품충적양육양본（산양、면양、잡교양등）균능특이확증출220～247 bp적목적편단，이저、우、계、압、토등동물양본균정음성。해방법대양육적검측민감도체10-10。응용해방법대산동성내도재장생산선、초시、시장중적각충축금선육양품진행감정，양육적검출솔위100％。
A pair of specific microsatellite molecular markers was screened according to the rule of spe -cies specificity to establish a PCR method for identifying mutton authenticity .With this method , 220~247 bp target fragment could be amplified from 11 sheep or goat breeds ,while nothing could be amplified from pigs , cattle, chickens, ducks and rabbits .The detecting sensitivity of this method could reach 10 -10 .With this method to identify carcass meats of livestock and poultry from slaughtering factories , supermarkets and ped-lars’ markets, the detectable rate was 100%.