CAJ | 학술논문

目的：探讨大鼠肝缺血/再灌注（I/R）后PEP-1介导血红素加氧酶-1（HO-1）对肝脏超氧化物歧化酶（SOD）、丙二醛（MDA）及caspase-3的影响。方法制作肝I/R损伤动物模型，SD大鼠随机分为4组，即假手术组（S组），肝缺血再灌注组（I/R组）、HO-1组、PEP-1-HO-1组。I/R后12 h光镜及电镜下观察肝细胞病理学改变，检测血清ALT的水平、肝组织MDA的含量及SOD的活性，免疫组化染色检测肝组织caspase-3的表达。结果 PEP-1-HO-1组血清ALT、肝组织MDA变化幅度明显低于I/R组，肝组织SOD明显高于I/R组（P ＜0．05）。在电镜下观察，l/R组肝小叶结构紊乱，肝窦淤血，肝细胞水肿变性，肝细胞片状坏死。HO-1组和PEP-1-HO-1组上述改变明显减轻。在I/R组中，caspase-3较S组表达增强，而在HO-1组、PEP-1-HO1组中其表达较I/R组减弱。结论 PEP-1介导HO-1对肝I/R损伤有保护作用，其作用机制可能与减少氧自由基产生、减轻脂质过氧化反应及抑制caspase-3的表达有关。
목적：탐토대서간결혈/재관주（I/R）후PEP-1개도혈홍소가양매-1（HO-1）대간장초양화물기화매（SOD）、병이철（MDA）급caspase-3적영향。방법제작간I/R손상동물모형，SD대서수궤분위4조，즉가수술조（S조），간결혈재관주조（I/R조）、HO-1조、PEP-1-HO-1조。I/R후12 h광경급전경하관찰간세포병이학개변，검측혈청ALT적수평、간조직MDA적함량급SOD적활성，면역조화염색검측간조직caspase-3적표체。결과 PEP-1-HO-1조혈청ALT、간조직MDA변화폭도명현저우I/R조，간조직SOD명현고우I/R조（P ＜0．05）。재전경하관찰，l/R조간소협결구문란，간두어혈，간세포수종변성，간세포편상배사。HO-1조화PEP-1-HO-1조상술개변명현감경。재I/R조중，caspase-3교S조표체증강，이재HO-1조、PEP-1-HO1조중기표체교I/R조감약。결론 PEP-1개도HO-1대간I/R손상유보호작용，기작용궤제가능여감소양자유기산생、감경지질과양화반응급억제caspase-3적표체유관。
Objective To investigate the effects of PEP-1-mediated heme oxygenase-1 (HO-1 )on su-peroxide dismutase(SOD)and malondialdehyde(MDA)and Caspase-3 in rats with hepatic ischemia-reperfusion (I/R)injury.Methods Sprague-Dawley rats were used establish models of I/R injury and randomly divided into four group:the sham-operation group (S group ),the hepatic I/R group,HO-1 group and PEP-1-HO-1 group.After 12h of reperfusion,liver tissues were harvested for the observation of pathological changes through light and electron microscope.The levels of serum ALT,hepatic MDA and SOD activity were tested.Expressions of hepatic Caspase-3 were tested with immunohistochemistry.Results Compared with the I/R group,the levels of serum ALT and hepatic MDA were significantly decreased with the increasing activity of SOD in the PEP-1-HO-1 group.Obvious I/R injury was observed under the electron microscope in the I/R group,including disor-dered structure of hepatic lobule,congestion in liver sinusoids,hydropic degeneration and patchy necrosis of hepatocyte.In the HO-1 group and PEP-1-HO-1 group,those hepatic tissue injuries were significantly improved. Comparing with the S group,the Caspase-3 expression in the I/R group increased and it's higher than those in the HO-1 group and PEP-1-HO-1 group.Conclusion PEP-1-mediated HO-1 protect liver from hepatic I/R in-jury.The mechanism of protection may be associated with the inhibition of oxygen radical,lipid peroxidation and Caspase-3 expression.