临床肝胆病杂志
臨床肝膽病雜誌
림상간담병잡지
CHINESE JOURNAL OF CLINICAL HEPATOLOGY
2014年
6期
546-548
,共3页
胆结石%肠黏膜%通透性
膽結石%腸黏膜%通透性
담결석%장점막%통투성
cholelithiasis%intestinal mucosa%permeability
目的：研究不同部位及性质胆石症患者肠黏膜通透性的改变，并探讨肠屏障功能在胆石形成中的作用。方法选择2011年3月-2013年3月收治的108例胆结石患者，健康对照者20例，按结石部位不同分为4组：健康对照组（A1组）、胆囊结石组（B1组）、胆管结石组（C1组）、胆囊结石合并胆管结石组（D1组）；所有入选患者全部行手术治疗，收集胆石进行化学分析，根据结石不同性质分为健康对照组（A2组）、胆固醇结石（B2组）、胆色素结石（C2组）、混合性结石组（D2组）。分别用分光光度法测定各组血浆及回肠末端黏膜组织中D-乳酸浓度、二胺氧化酶（DAO）活性，组间比较采用方差分析和LSD-t检验。结果（1）不同部位结石患者（A1、B1、C1、D14组）之间血浆及肠黏膜组织D-乳酸及DAO活性水平相比较差异无统计学意义（P＞0．05）；（2）术后对胆石进行化学分析，胆固醇结石40例（37．04％）、胆色素结石52例（48．15％），混合性结石16例（14．81％）；与A2组及B2组比较，C2组血浆及肠黏膜组织D-乳酸及DAO活性差异有统计学意义（P＜0．05），与D2组比较，差异无统计学意义（P＞0．05）；B2组与A2、D2组比较，差异无统计学意义（P＞0．05）。结论胆色素结石患者存在肠黏膜通透性的改变，胆色素结石形成与肠屏障功能损伤有一定的关系，肠屏障功能损伤可能在促进胆色素结石的形成中发挥一定的作用。
目的：研究不同部位及性質膽石癥患者腸黏膜通透性的改變，併探討腸屏障功能在膽石形成中的作用。方法選擇2011年3月-2013年3月收治的108例膽結石患者，健康對照者20例，按結石部位不同分為4組：健康對照組（A1組）、膽囊結石組（B1組）、膽管結石組（C1組）、膽囊結石閤併膽管結石組（D1組）；所有入選患者全部行手術治療，收集膽石進行化學分析，根據結石不同性質分為健康對照組（A2組）、膽固醇結石（B2組）、膽色素結石（C2組）、混閤性結石組（D2組）。分彆用分光光度法測定各組血漿及迴腸末耑黏膜組織中D-乳痠濃度、二胺氧化酶（DAO）活性，組間比較採用方差分析和LSD-t檢驗。結果（1）不同部位結石患者（A1、B1、C1、D14組）之間血漿及腸黏膜組織D-乳痠及DAO活性水平相比較差異無統計學意義（P＞0．05）；（2）術後對膽石進行化學分析，膽固醇結石40例（37．04％）、膽色素結石52例（48．15％），混閤性結石16例（14．81％）；與A2組及B2組比較，C2組血漿及腸黏膜組織D-乳痠及DAO活性差異有統計學意義（P＜0．05），與D2組比較，差異無統計學意義（P＞0．05）；B2組與A2、D2組比較，差異無統計學意義（P＞0．05）。結論膽色素結石患者存在腸黏膜通透性的改變，膽色素結石形成與腸屏障功能損傷有一定的關繫，腸屏障功能損傷可能在促進膽色素結石的形成中髮揮一定的作用。
목적：연구불동부위급성질담석증환자장점막통투성적개변，병탐토장병장공능재담석형성중적작용。방법선택2011년3월-2013년3월수치적108례담결석환자，건강대조자20례，안결석부위불동분위4조：건강대조조（A1조）、담낭결석조（B1조）、담관결석조（C1조）、담낭결석합병담관결석조（D1조）；소유입선환자전부행수술치료，수집담석진행화학분석，근거결석불동성질분위건강대조조（A2조）、담고순결석（B2조）、담색소결석（C2조）、혼합성결석조（D2조）。분별용분광광도법측정각조혈장급회장말단점막조직중D-유산농도、이알양화매（DAO）활성，조간비교채용방차분석화LSD-t검험。결과（1）불동부위결석환자（A1、B1、C1、D14조）지간혈장급장점막조직D-유산급DAO활성수평상비교차이무통계학의의（P＞0．05）；（2）술후대담석진행화학분석，담고순결석40례（37．04％）、담색소결석52례（48．15％），혼합성결석16례（14．81％）；여A2조급B2조비교，C2조혈장급장점막조직D-유산급DAO활성차이유통계학의의（P＜0．05），여D2조비교，차이무통계학의의（P＞0．05）；B2조여A2、D2조비교，차이무통계학의의（P＞0．05）。결론담색소결석환자존재장점막통투성적개변，담색소결석형성여장병장공능손상유일정적관계，장병장공능손상가능재촉진담색소결석적형성중발휘일정적작용。
Objective To study the changes in intestinal permeability in patients with gallstones of different distribution and properties and to in-vestigate the role of intestinal barrier function in gallstone formation.Methods A total of 108 patients with gallstones in our hospital in need of op-eration from March 2011 to March 2013 were enrolled in this study.Twenty healthy controls were also included.According to the distribution of gallstones,the subjects were separated into the following four groups:normal control group (group A1),gallbladder stone group (group B1),bile duct stone group (group C1),and combined group (group D1),in which gallbladder stones were complicated by bile duct stones.All patients un-derwent operation,and gallstones were collected for chemical analysis.According to the chemical properties of gallstones,the subjects were re-grouped as follows:normal control group (group A2),cholesterol stone group (group B2),pigment stone group (group C2),and mixed stone group (group D2),in which cholesterol stones were mixed with pigment stones.D-lactic acid level and diamine oxidase (DAO)activity in plas-ma and mucosal tissue of the terminal ileum were measured by spectrophotometry.Differences were analysed by ANOVA and LSD t-test.Results Plasma D-lactic acid level and intestinal DAO activity showed no significant differences between groups A1,B1,C1,and D1 (P>0.05). Chemical analysis after surgery found 40 cases of cholesterol stones (37.04%),52 cases of pigment stones (48.15%),and 16 cases of mixed stones (14.8%).Group C2 showed significantly different D-lactic acid level and DAO activity compared with B2 and A2 (P<0.05 ),but no differences from D2 (P>0.05).Group B2 was found of no differences from A2 and D2 (P>0.05).Conclusion Patients with pigment stones were all found of changes in intestinal permeability.The formation of pigment stones is related to impairment of intestinal barrier function.It is possible that impairment of intestinal barrier function plays a certain role in pigment stone formation.
