CAJ | 학술논문

目的：探讨鱼腥草及三丫苦对甲型流感病毒（nucleo protein antigen，NPAG）表达的影响，了解鱼腥草及三丫苦抗甲型流感病毒的分子机制。方法以胶体金法测定NPAG的表达。瞬间转染，实验分为：Hela细胞组、空质粒组[PCDNA3.1(+)/empty]、重组质粒组[PCDNA3.1(+)/NP)]、脂质体组、三丫苦醇提物组和鱼腥草油组。药物实验加入重组质粒同时将药物加入培养细胞孔中进行干预。转染48h后，测定其上清液NPAG的表达。结果胶体金法测试显示：Hela细胞组、PCDNA3.1(+)/empty组、脂质体组、鱼腥草油组为阴性；PCDNA3.1(+)/NP组、三丫苦醇提物为阳性，NPAG浓度1：64。结论鱼腥草油组可抑制甲型流感病毒NPAG的表达，能抗甲型流感病毒。三丫苦醇提物组不影响NPAG的表达。
목적：탐토어성초급삼아고대갑형류감병독（nucleo protein antigen，NPAG）표체적영향，료해어성초급삼아고항갑형류감병독적분자궤제。방법이효체금법측정NPAG적표체。순간전염，실험분위：Hela세포조、공질립조[PCDNA3.1(+)/empty]、중조질립조[PCDNA3.1(+)/NP)]、지질체조、삼아고순제물조화어성초유조。약물실험가입중조질립동시장약물가입배양세포공중진행간예。전염48h후，측정기상청액NPAG적표체。결과효체금법측시현시：Hela세포조、PCDNA3.1(+)/empty조、지질체조、어성초유조위음성；PCDNA3.1(+)/NP조、삼아고순제물위양성，NPAG농도1：64。결론어성초유조가억제갑형류감병독NPAG적표체，능항갑형류감병독。삼아고순제물조불영향NPAG적표체。
Objective To investigated the effect of Yuxincao and Sanyaku on expression of influenza a virus nucleoprotein antigen (NPAG),Mechanism of Yuxincao and Sanyaku ant influenza a virus on the molecular level. Methods Colloidal gold im-munochromatography methods using to measure NPAG expression of Hela cell group,PCDNA3.1(+)/empty group,PCDNA3.1(+)/NP group, liposome group,Sanyaku group and Yuxincao group. the successfully transfected HeLa cells as target cells. 48 hours after transfection,the supernatant was used to test the expression of NPAG. Results Collodial gold showed Hela cell group and PCD-NA3.1(+)/empty group were negative,PCDNA3.1(+)/NP group and Sanyaku group were NPAG positive. Titration of NPAG were 1:64. Conclusion Yuxincao group could inhibit NPAG expression of influnte A Virus,Sanyaku group not could inhibit.