CAJ | 학술논문

目的：探讨急性心肌梗死合并室颤与MMP-9表达的相关性。方法选择我院10例急性心肌梗死合并室颤患者纳入实验组；同期选择本院10例急性心肌梗死未合并室颤患者作为对照组。实验组及对照组于发病后第1、3、5d各抽取外周静脉血5ml，-20℃冰箱保存。采用RT-PCR 及ELISA 法同时测定MMP-9。结果 RT-PCR 结果显示实验组第1d血细胞MMP-9基因表达比对照组高1.73倍（1.03-2.96）；实验组第3d MMP-9基因表达比对照组高1.54倍（1.09-2.92）；实验组第5d MMP-9基因表达比对照组高1.61倍（1.07-3.09）。实验组第1d与实验组第3、5d MMP-9表达ΔCT差异具有统计学意义（均P<0.05）。对照组第1、3、5d MMP-9组间基因表达ΔCT值差异具有统计学意义（均P<0.05），对照组第1d与对照组第3、5d的 MMP-9表达差异具有统计学意义（ΔCT比较，均P<0.05）。 ELISA结果显示实验组第1d、3d、5d MMP-9水平显著高于对照组同时间点MMP-9水平（均P<0.05）。对照组第3、5d血清 MMP-9水平显著高于对照组第1d 血清MMP-9水平（均P<0.05）。实验组第3、5d血清MMP-9水平显著高于实验组第1d 血清MMP-9水平（均P<0.05）。结论 MMP-9在急性心肌梗死合并室颤发生、发展中起到促进作用，增加了急性心肌梗死患者发生室颤可能性。
목적：탐토급성심기경사합병실전여MMP-9표체적상관성。방법선택아원10례급성심기경사합병실전환자납입실험조；동기선택본원10례급성심기경사미합병실전환자작위대조조。실험조급대조조우발병후제1、3、5d각추취외주정맥혈5ml，-20℃빙상보존。채용RT-PCR 급ELISA 법동시측정MMP-9。결과 RT-PCR 결과현시실험조제1d혈세포MMP-9기인표체비대조조고1.73배（1.03-2.96）；실험조제3d MMP-9기인표체비대조조고1.54배（1.09-2.92）；실험조제5d MMP-9기인표체비대조조고1.61배（1.07-3.09）。실험조제1d여실험조제3、5d MMP-9표체ΔCT차이구유통계학의의（균P<0.05）。대조조제1、3、5d MMP-9조간기인표체ΔCT치차이구유통계학의의（균P<0.05），대조조제1d여대조조제3、5d적 MMP-9표체차이구유통계학의의（ΔCT비교，균P<0.05）。 ELISA결과현시실험조제1d、3d、5d MMP-9수평현저고우대조조동시간점MMP-9수평（균P<0.05）。대조조제3、5d혈청 MMP-9수평현저고우대조조제1d 혈청MMP-9수평（균P<0.05）。실험조제3、5d혈청MMP-9수평현저고우실험조제1d 혈청MMP-9수평（균P<0.05）。결론 MMP-9재급성심기경사합병실전발생、발전중기도촉진작용，증가료급성심기경사환자발생실전가능성。
Objective To expolore the relevance of MMP-9 in acute myocardial infarction merger ventricular fibrillation. Methods 10 cases of acute myocardial infarction in our hospital patients with combination of ventricular fibrillation were enrolled as the experimental group;The same choice in 10 cases of acute myocardial infarction patients without combination of ventricular fibrillation were enrolled as control group. The experimental group and control group in incidence after 1,3,5d each extraction of peripheral venous blood 5ml,-20℃ refrigerator. By RT-PCR and elisa method and determine the MMP-9. Results RT-PCR re-sults show that the experimental group 1d cells MMP-9 gene expression was 1.73 times higher than the control group (1.03 2.96);Group 3d MMP-9 gene expression was 1.54 times higher than the control group(1.09 2.92);Group 5d MMP-9 gene expression was 1.61 times higher than the control group(1.07 3.09). Experimental group 1d and 3,5d MMP-9ΔCT expression was differences sta-tistically significant(all P<0.05). Control group 1,3,5d gene expression between MMP-9 groups ΔCT value was differences statisti-cally significant (P<0.05),control group in 1d and control group in 3,5d of MMP-9 expression was differences statistically signifi-cant(ΔCT,P<0.05). Elisa results showed that the experimental group 1,3,5d level of MMP-9 was significantly higher than control group at the same time some level of MMP-9 (P<0.05). Control group in 3,5d was significantly higher serum levels of MMP-9 1d serum level of MMP-9 (P<0.05). Group 3,5d were significantly higher serum levels of MMP-9 experimental group 1d serum level of MMP-9 (P<0.05). Conclusion MMP-9 in acute myocardial infarction merger ventricular fibrillation play a promoting role in occurrence and development,increase the possibility ventricular fibrillation in patients with acute myocardial infarction.