CAJ | 학술논문

基于哺乳动物脑组织含有丰富的乙酰胆碱酯酶这一生物学、医药学常识，采用新鲜的猪脑膜作为酶膜，将其紧贴于pH玻璃电极玻璃球敏感膜上作为酶源，构建电化学生物传感器。该传感器对氯化乙酰胆碱具有灵敏的响应。对测定体系的缓冲溶液的pH进行了优化，考察了该传感器的选择性和寿命，并研究了几种与水混溶的有机溶剂对酶相对活性的影响以及传感器在有机溶剂中的稳定性。基于氨基甲酸酯类农药会对乙酰胆碱酯酶发生抑制作用这一机理，选择最佳浓度的有机溶剂作为测试介质，将该传感器用于氨基甲酸酯类农药西维因的测定，具有线性范围广、检测限低、重现性较好的特点。应用于城市污水处理厂的出厂水中西维因的测定，回收率在92．5%~105．0%之间，具有实用价值。
기우포유동물뇌조직함유봉부적을선담감지매저일생물학、의약학상식，채용신선적저뇌막작위매막，장기긴첩우pH파리전겁파리구민감막상작위매원，구건전화학생물전감기。해전감기대록화을선담감구유령민적향응。대측정체계적완충용액적pH진행료우화，고찰료해전감기적선택성화수명，병연구료궤충여수혼용적유궤용제대매상대활성적영향이급전감기재유궤용제중적은정성。기우안기갑산지류농약회대을선담감지매발생억제작용저일궤리，선택최가농도적유궤용제작위측시개질，장해전감기용우안기갑산지류농약서유인적측정，구유선성범위엄、검측한저、중현성교호적특점。응용우성시오수처리엄적출엄수중서유인적측정，회수솔재92．5%~105．0%지간，구유실용개치。
Based on the general knowledge of biology and medicine that acetylcholinesterase mainly exist in animal’ s brain tissue, we choose the fresh swinish brain membrane containing acetylcholinesterase as the enzyme membrane directly attached to the pH glass electrode to construct the biosensor. The biosensor responded sensitively to acetylcholine. Optimization of buffers’ pH and con-centration were studied,as well as the selectivity and life-time of the biosensor. The influence of various concentrations of water-mis-cible organic solvents on the response of the biosensor and its operating stability were also investigated. Choosing the optimum con-centration of water-miscible organic solvents as test medium,the biosensor was applied to detect carbaryl,one of the typic carbamate pesticides. It showed wide linearity range,low detection limit and good repeatability. The recovery percentages ranged from 92. 5%to 105. 0%for determining carbaryl of domestic wastewater treated by municipal sewage treatment plants. The biosensor is suitable for real application.