化学研究与应用
化學研究與應用
화학연구여응용
CHEMICAL RESEARCH AND APPLICATION
2014年
6期
804-808
,共5页
牛血清蛋白%硝基苯%相互作用%荧光猝灭
牛血清蛋白%硝基苯%相互作用%熒光猝滅
우혈청단백%초기분%상호작용%형광졸멸
bovine serum albumin%nitrobenzene%interaction%fluorescence quenching
本文应用荧光分析法研究了不同酸度、温度和反应时间条件下,硝基苯对牛血清蛋白( BSA)的荧光猝灭作用。实验结果表明在激发波长λex=280nm,发射波长λem=342nm,浓度为0.05 mol·L-1,pH=7.50的Tris-HCl缓冲溶液中,猝灭效果最为明显。计算289,304和318 K 温度下二者的结合常数分别为:1.25×104、1.00×104和0.833×104 L·mol-1。通过Gibbs-Helmholtz 方程对其相互作用的热力学参数进行计算(ΔH=-10.7 kJ· moL-1;ΔS=41.4 J·moL-1·K-1),表明二者之间是静电相互作用。实验结果表明,硝基苯对牛血清蛋白荧光猝灭方式为静态猝灭,最后使用紫外吸收光谱法对其作用机理进一步确认。
本文應用熒光分析法研究瞭不同痠度、溫度和反應時間條件下,硝基苯對牛血清蛋白( BSA)的熒光猝滅作用。實驗結果錶明在激髮波長λex=280nm,髮射波長λem=342nm,濃度為0.05 mol·L-1,pH=7.50的Tris-HCl緩遲溶液中,猝滅效果最為明顯。計算289,304和318 K 溫度下二者的結閤常數分彆為:1.25×104、1.00×104和0.833×104 L·mol-1。通過Gibbs-Helmholtz 方程對其相互作用的熱力學參數進行計算(ΔH=-10.7 kJ· moL-1;ΔS=41.4 J·moL-1·K-1),錶明二者之間是靜電相互作用。實驗結果錶明,硝基苯對牛血清蛋白熒光猝滅方式為靜態猝滅,最後使用紫外吸收光譜法對其作用機理進一步確認。
본문응용형광분석법연구료불동산도、온도화반응시간조건하,초기분대우혈청단백( BSA)적형광졸멸작용。실험결과표명재격발파장λex=280nm,발사파장λem=342nm,농도위0.05 mol·L-1,pH=7.50적Tris-HCl완충용액중,졸멸효과최위명현。계산289,304화318 K 온도하이자적결합상수분별위:1.25×104、1.00×104화0.833×104 L·mol-1。통과Gibbs-Helmholtz 방정대기상호작용적열역학삼수진행계산(ΔH=-10.7 kJ· moL-1;ΔS=41.4 J·moL-1·K-1),표명이자지간시정전상호작용。실험결과표명,초기분대우혈청단백형광졸멸방식위정태졸멸,최후사용자외흡수광보법대기작용궤리진일보학인。
By using fluorescence spectroscopy,the interaction between nitrobenzene( NB) and bovine serum albumin( BSA) has been discussed in this paper with different experimental conditions,including acidity,temperature and reaction time. The results showed that the best quenching effect of NB on BSA occurred in 0. 05 mol·L-1Tris-HCl buffer solutions(pH 7. 50)when the optimum exci-tation and emission wavelength were 280 nm and 342 nm,respectively. The binding constants(KB)for NB and BSA at 289,304 and 318K are 1. 25 × 104 ,1. 00 × 104 and 0. 833 × 104 L·mol-1 , respectively. The thermodynamic parameters,ΔH calculated by using Gibbs-Helmholtz Equation is-10. 7 kJ·mol-1 and ΔS is 41. 4 J·mol-1·K-1 . The interactions between NB and BSA is ascribed to the electrostatic force. The experimental results demonstrated that the fluorescence quenching mechanism is static quenching,which has been further confirmed by ultraviolet absorption spectrometry.