CAJ | 학술논문

利用常压室温等离子体射流诱变和紫外照射对夫西地酸生产菌株进行复合诱变，得到3株产量明显提高的突变菌株，3株菌的平均发酵效价较出发菌株提高14％；然后，采用均匀设计实验对其中发酵效价提高最多的 AU-37菌株的发酵培养基碳、氮源进行了优化，得到适合该菌株的发酵培养基优化配方为：糊精1.86％、花生饼粉2.5％、蛋白胨0.3％、氯化铵0.5％、七水合硫酸镁0.3％、硫酸钾0.6％、碳酸钙1％、pH 值7.2，在优化的发酵培养基中，AU-37菌株摇瓶发酵效价较出发菌株提高33.8％，为工业化生产奠定了基础。
이용상압실온등리자체사류유변화자외조사대부서지산생산균주진행복합유변，득도3주산량명현제고적돌변균주，3주균적평균발효효개교출발균주제고14％；연후，채용균균설계실험대기중발효효개제고최다적 AU-37균주적발효배양기탄、담원진행료우화，득도괄합해균주적발효배양기우화배방위：호정1.86％、화생병분2.5％、단백동0.3％、록화안0.5％、칠수합류산미0.3％、류산갑0.6％、탄산개1％、pH 치7.2，재우화적발효배양기중，AU-37균주요병발효효개교출발균주제고33.8％，위공업화생산전정료기출。
Fusidic acid-producing strain FA-30 was mutated by ARTP-UV composite mutagenesis,three high-efficient producing strains were obtained by screening,which was increased in fermentation potency by 14% over the original strain.The carbon source and nitrogen source of the fermentation medium for strain AU-37 with the highest fermentation potency were optimized through the uniform design experiment.The optimum fermentation medium was as follows:dextrin 1.86%,peanut cake powder 2.5%,peptone 0.3%,NH 4 Cl 0.5%, MgSO4 ·7H2 O 0.3%,K2 SO4 0.6%,CaCO3 1%,pH value 7.2,and the fermentation potency of strain AU-37 cultured in the above medium reached 33.8% over that of the original strain.