CAJ | 학술논문

以水栀子果实为原料、水为提取溶剂，采用大孔树脂吸附分离技术富集纯化栀子黄色素。结果表明，选取HPD100A 大孔树脂作为吸附剂，将3级提取液过滤除渣后分别以10 BV·h-1、30 BV·h-1、60 BV·h-1（BV 表示柱体积）的速率通过树脂柱，树脂吸附饱和后，先用水洗，然后用70％乙醇以0.5 BV·h-1洗脱收集，浓缩干燥后得纯化的栀子黄色素，其色价达384.3±4.3、OD 比值为0.34±0.004。对栀子黄色素的稳定性进行考察发现：使用时，溶液应控制pH 值4～10，现配现用；储存时，宜避光、低温。
이수치자과실위원료、수위제취용제，채용대공수지흡부분리기술부집순화치자황색소。결과표명，선취HPD100A 대공수지작위흡부제，장3급제취액과려제사후분별이10 BV·h-1、30 BV·h-1、60 BV·h-1（BV 표시주체적）적속솔통과수지주，수지흡부포화후，선용수세，연후용70％을순이0.5 BV·h-1세탈수집，농축간조후득순화적치자황색소，기색개체384.3±4.3、OD 비치위0.34±0.004。대치자황색소적은정성진행고찰발현：사용시，용액응공제pH 치4～10，현배현용；저존시，의피광、저온。
The study focused on the enrichment and purification of gardenia yellow pigment from the fruit of Gardenia jasminoides var.radicans Makino by macroporous resin.The results showed that the HPD100A macroporous resin was chosen to purify gardenia yellow pigment because of its high efficiency of adsorption and desorption as well as its high selectivity,and the three kinds of crude extraction solution passed the resin at the flow rate of 10 BV·h-1 ,30 BV·h-1 ,60 BV·h-1 (BV represents column volume)after filtration,respectively. The resin column was washed with water firstly,and then desorbed with ethanol with volume fraction of 70% at the speed of 0.5 BV·h-1 .The pigment solution was collected,concentrated and dried ,then the product was obtained with the colority of 384.3±4.3 and the OD ratio of 0.35±0.004.The study on the stability of garde-nia yellow pigment showed that:the pH value of gardenia yellow pigment solution should be controlled between 4 and 10,and the solution should be used as soon as possible,while a dark and cool place should be provided to the product when stored.