CAJ | 학술논문

用 Isobologram 分析和清除·DPPH 法相结合的方法研究拳参黄酮与 VC、芦丁的协同抗氧化作用。结果表明：VC、芦丁和拳参黄酮清除·DPPH 能力大小为：VC＞拳参黄酮＞芦丁；由 Isobologram 分析得到复配后的效应点均在95％置信区和相加线的左侧，理论 IC50add与实验 IC50mix有显著差异，相互作用指数γ均小于1；拳参黄酮与 VC 或芦丁复配均可以增强抗氧化作用效果，比任一单独作用效果更好。
용 Isobologram 분석화청제·DPPH 법상결합적방법연구권삼황동여 VC、호정적협동항양화작용。결과표명：VC、호정화권삼황동청제·DPPH 능력대소위：VC＞권삼황동＞호정；유 Isobologram 분석득도복배후적효응점균재95％치신구화상가선적좌측，이론 IC50add여실험 IC50mix유현저차이，상호작용지수γ균소우1；권삼황동여 VC 혹호정복배균가이증강항양화작용효과，비임일단독작용효과경호。
The synergistic antioxidant activities of flavonoids from Polygonum bistorta L.combined with VC or rutin,were investigated by evaluating their ability to scavenge ·DPPH with Isobologram analysis.The capacity of scavenging·DPPH was as follows:VC>flavonoids from Polygonum bistorta L.>rutin.And Isobo-logram analysis demonstrated the response factor of flavonoids from Polygonum bistorta L.combined with VC or rutin were both located in the left of the addition line and 95% confidence interval.There was a significant difference between theoretical IC50add value and experimental IC50mix value,for which the interaction index(γ)was less than 1.The results showed flavonoids from Polygonum bistorta L.combined with VC or rutin exhibited stronger antioxidant activity than that of the single one.