动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2014年
6期
171-175
,共5页
伪狂犬病病毒%猪瘟病毒%混合感染%诊断
偽狂犬病病毒%豬瘟病毒%混閤感染%診斷
위광견병병독%저온병독%혼합감염%진단
Pseudorabies virus%Classical swine fever virus%mixed infection%diagnosis
山东某猪场发病猪数头，经症状观察与临床剖检，结合当地流行病学调查及该猪场免疫情况，初步诊断为猪伪狂犬病病毒（Pseudorabies virus，PRV）与猪瘟病毒（Classical swine fever virus，CSFV）混合感染。因此采集患病猪只的病变组织，进行实验室诊断。将病变组织按常规方法处理后，提取总 DNA 与RNA，经 PCR 扩增，获得 PRV 阳性目的片段约217 bp；经 RT-PCR 扩增，扩增出 CSFV 阳性片段约510 bp；证明该猪场为 PRV 与 CSFV 混合感染。随后应用 BHK-21细胞与 PK-15细胞分别进行 PRV 与 CSFV 的分离培养，并盲传3代。结果显示，经 BHK-21细胞传代培养至 F3代，可见典型的 PRV 细胞病变，经 PCR进一步鉴定为 PRV，毒力测定其 TCID50为106．68 TCID50/0．1 mL；PK-15细胞传代培养至 F3代，经 PCR 鉴定为 CSFV 阳性。综合以上结果，最终确诊该猪场为 CSFV 与 PRV 混合感染。随后采用紧急接种的预防方法，并进行患病猪只的部分淘汰，最终获得了满意的防控效果，降低了经济损失。
山東某豬場髮病豬數頭，經癥狀觀察與臨床剖檢，結閤噹地流行病學調查及該豬場免疫情況，初步診斷為豬偽狂犬病病毒（Pseudorabies virus，PRV）與豬瘟病毒（Classical swine fever virus，CSFV）混閤感染。因此採集患病豬隻的病變組織，進行實驗室診斷。將病變組織按常規方法處理後，提取總 DNA 與RNA，經 PCR 擴增，穫得 PRV 暘性目的片段約217 bp；經 RT-PCR 擴增，擴增齣 CSFV 暘性片段約510 bp；證明該豬場為 PRV 與 CSFV 混閤感染。隨後應用 BHK-21細胞與 PK-15細胞分彆進行 PRV 與 CSFV 的分離培養，併盲傳3代。結果顯示，經 BHK-21細胞傳代培養至 F3代，可見典型的 PRV 細胞病變，經 PCR進一步鑒定為 PRV，毒力測定其 TCID50為106．68 TCID50/0．1 mL；PK-15細胞傳代培養至 F3代，經 PCR 鑒定為 CSFV 暘性。綜閤以上結果，最終確診該豬場為 CSFV 與 PRV 混閤感染。隨後採用緊急接種的預防方法，併進行患病豬隻的部分淘汰，最終穫得瞭滿意的防控效果，降低瞭經濟損失。
산동모저장발병저수두，경증상관찰여림상부검，결합당지류행병학조사급해저장면역정황，초보진단위저위광견병병독（Pseudorabies virus，PRV）여저온병독（Classical swine fever virus，CSFV）혼합감염。인차채집환병저지적병변조직，진행실험실진단。장병변조직안상규방법처리후，제취총 DNA 여RNA，경 PCR 확증，획득 PRV 양성목적편단약217 bp；경 RT-PCR 확증，확증출 CSFV 양성편단약510 bp；증명해저장위 PRV 여 CSFV 혼합감염。수후응용 BHK-21세포여 PK-15세포분별진행 PRV 여 CSFV 적분리배양，병맹전3대。결과현시，경 BHK-21세포전대배양지 F3대，가견전형적 PRV 세포병변，경 PCR진일보감정위 PRV，독력측정기 TCID50위106．68 TCID50/0．1 mL；PK-15세포전대배양지 F3대，경 PCR 감정위 CSFV 양성。종합이상결과，최종학진해저장위 CSFV 여 PRV 혼합감염。수후채용긴급접충적예방방법，병진행환병저지적부분도태，최종획득료만의적방공효과，강저료경제손실。
The swine were initially diagnosed as mixed infection with Porcine pseudorabies virus (PRV)and Classical swine fever virus (CSFV)in a farm of Shandong through the clinical examination and combining with the local epidemiology and the vaccine inoculation status.Therefore,the lesion tissues of sick swine were collected and treated by routine method in laboratory.Sequentially,the total DNA and RNA were extracted and analyzed by PCR.The 217 bp fragment and 510 bp fragment were obtained through PCR and RT-PCR,that demenstrated that the swine co-infected with PRV and CSFV in the farm.The PRV and CSFV were isolated and cultured in BHK-21 cells and PK-15 cells independently.The results showed that BHK-21 cells displayed the typical PRV CPE and the cell cultures were further identified as PRV positive by PCR technology.Furthermore,the TCID50 of PRV cell cultures was determined as 10 6.68 TCID50/0.1 mL.On the other hand,the cell cultures were identified as CSFV positive after three passages since the li-sions were inoculated in PK-15 cells.Based on the above results,the swine in the farm were diagnosed as CSFV and PRV mixed infection finally.The swine were inoculated with PRV and CSFV emergently and some swine were emilinated.The curative effect was satisfactory,and the economic loss was reduced .
