CAJ | 학술논문

戊型肝炎是一种重要的人兽共患传染病，其病原戊型肝炎病毒（HEV）变异性强、宿主范围广。HEV 体外培养难度大，建立感染模型对深入研究 HEV 的复制机理、致病性和跨种系传播等具有重大意义。资料表明恒河猴、黑猩猩、短尾猴等非人灵长类，猪、兔等动物以及传代细胞等均有望作为感染模型。恒河猴、黑猩猩是理想的动物模型，但存在价格高、操作难和医学伦理等问题。猪、兔相对成本低，操作简便，但只感染基因3/4型。动物感染模型中，以长爪沙鼠较为理想。人胚肺二倍体传代细胞系、猪肝脏干细胞系、人肝癌细胞系和肺癌细胞系等均可用于 HEV 培养，以人肝癌细胞系 PLC/PRF/5和肺癌细胞系 A549最为高效。
무형간염시일충중요적인수공환전염병，기병원무형간염병독（HEV）변이성강、숙주범위엄。HEV 체외배양난도대，건립감염모형대심입연구 HEV 적복제궤리、치병성화과충계전파등구유중대의의。자료표명항하후、흑성성、단미후등비인령장류，저、토등동물이급전대세포등균유망작위감염모형。항하후、흑성성시이상적동물모형，단존재개격고、조작난화의학윤리등문제。저、토상대성본저，조작간편，단지감염기인3/4형。동물감염모형중，이장조사서교위이상。인배폐이배체전대세포계、저간장간세포계、인간암세포계화폐암세포계등균가용우 HEV 배양，이인간암세포계 PLC/PRF/5화폐암세포계 A549최위고효。
Hepatitis E is an important zoonotic disease caused by hepatitis E virus (HEV)that has high di-versity and many hosts.Due to difficult culture in vitro ,the development of HEV infection model for in-depth study is essential.the establish of an ideal infection model definitely will benefit the study on mecha-nism of replication,pathogenicity and cross-species infection of HEV.Animal models like nonhuman pri-mates,pigs,rabbits and cell culture system are possibly used as infection models.Nonhuman primates are practical models but limitation on cost,availability,manipulate and housing.Pigs and rabbits are relative-ly low cost,easy to manipulate,but only susceptible to genotype 3 and/or type 4.Among these animal model,Mongolian gerbil is the most practical at present.Cell culture systems have now become available, human hepatocellular carcinoma cell line PLC/PRF/5 and lung cancer cell A549 are the most effective cul-ture system.