动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2014年
6期
103-106
,共4页
李文贵%段新慧%张佳%严红亚
李文貴%段新慧%張佳%嚴紅亞
리문귀%단신혜%장가%엄홍아
戊型肝炎病毒%感染模型%宿主
戊型肝炎病毒%感染模型%宿主
무형간염병독%감염모형%숙주
Hepatitis E virus%infection model%host
戊型肝炎是一种重要的人兽共患传染病,其病原戊型肝炎病毒(HEV)变异性强、宿主范围广。HEV 体外培养难度大,建立感染模型对深入研究 HEV 的复制机理、致病性和跨种系传播等具有重大意义。资料表明恒河猴、黑猩猩、短尾猴等非人灵长类,猪、兔等动物以及传代细胞等均有望作为感染模型。恒河猴、黑猩猩是理想的动物模型,但存在价格高、操作难和医学伦理等问题。猪、兔相对成本低,操作简便,但只感染基因3/4型。动物感染模型中,以长爪沙鼠较为理想。人胚肺二倍体传代细胞系、猪肝脏干细胞系、人肝癌细胞系和肺癌细胞系等均可用于 HEV 培养,以人肝癌细胞系 PLC/PRF/5和肺癌细胞系 A549最为高效。
戊型肝炎是一種重要的人獸共患傳染病,其病原戊型肝炎病毒(HEV)變異性彊、宿主範圍廣。HEV 體外培養難度大,建立感染模型對深入研究 HEV 的複製機理、緻病性和跨種繫傳播等具有重大意義。資料錶明恆河猴、黑猩猩、短尾猴等非人靈長類,豬、兔等動物以及傳代細胞等均有望作為感染模型。恆河猴、黑猩猩是理想的動物模型,但存在價格高、操作難和醫學倫理等問題。豬、兔相對成本低,操作簡便,但隻感染基因3/4型。動物感染模型中,以長爪沙鼠較為理想。人胚肺二倍體傳代細胞繫、豬肝髒榦細胞繫、人肝癌細胞繫和肺癌細胞繫等均可用于 HEV 培養,以人肝癌細胞繫 PLC/PRF/5和肺癌細胞繫 A549最為高效。
무형간염시일충중요적인수공환전염병,기병원무형간염병독(HEV)변이성강、숙주범위엄。HEV 체외배양난도대,건립감염모형대심입연구 HEV 적복제궤리、치병성화과충계전파등구유중대의의。자료표명항하후、흑성성、단미후등비인령장류,저、토등동물이급전대세포등균유망작위감염모형。항하후、흑성성시이상적동물모형,단존재개격고、조작난화의학윤리등문제。저、토상대성본저,조작간편,단지감염기인3/4형。동물감염모형중,이장조사서교위이상。인배폐이배체전대세포계、저간장간세포계、인간암세포계화폐암세포계등균가용우 HEV 배양,이인간암세포계 PLC/PRF/5화폐암세포계 A549최위고효。
Hepatitis E is an important zoonotic disease caused by hepatitis E virus (HEV)that has high di-versity and many hosts.Due to difficult culture in vitro ,the development of HEV infection model for in-depth study is essential.the establish of an ideal infection model definitely will benefit the study on mecha-nism of replication,pathogenicity and cross-species infection of HEV.Animal models like nonhuman pri-mates,pigs,rabbits and cell culture system are possibly used as infection models.Nonhuman primates are practical models but limitation on cost,availability,manipulate and housing.Pigs and rabbits are relative-ly low cost,easy to manipulate,but only susceptible to genotype 3 and/or type 4.Among these animal model,Mongolian gerbil is the most practical at present.Cell culture systems have now become available, human hepatocellular carcinoma cell line PLC/PRF/5 and lung cancer cell A549 are the most effective cul-ture system.