CAJ | 학술논문

为观察 N-乙酰半胱氨酸（NAC）抗氧化作用对慢性阻塞性肺疾病（COPD）模型大鼠血管活性物质的影响，将100只清洁级 Wistar 大鼠随机分为对照组、模型组、NAC 低剂量组、中剂量、高剂量5组，每组20只。模型组采用气管注入脂多糖（LPS）和香烟染毒的复合方法造模。在模型组基础上，低剂量组、中剂量、高剂量组分别按26．32、52．64、105．28 mg/kg NAC 灌胃，连续2周。检测丙二醛（MDA）、超氧化物歧化酶（SOD）、血管紧张素Ⅱ（AngⅡ）、活性氧（ROS）、内皮素（ET）、一氧化氮（NO）及肺组织核转录因子κB （NF-κB）的变化，分析 AngⅡ与 ROS、NF-κB 的相关性。结果显示，与对照组比较，模型组 AngⅡ、ROS、ET、MDA 水平均显著升高而 NO、SOD 水平极显著下降（P＜0．01），NF-κB 活性极显著升高（P＜0．01）。与模型组比较，低剂量组 NO 水平升高而 ET 水平显著下降（P＜0．05）；中剂量组 AngⅡ、ROS、ET、MDA 水平下降或显著下降而 NO、SOD 水平均升高（P ＜0．05或 P ＜0．01），NF-κB 活性显著下降（P ＜0．05）；高剂量组AngⅡ、ROS、ET、MDA 水平均显著下降而 NO、SOD 水平均极显著升高（P ＜0．01），NF-κB 活性极显著下降（P ＜0．01）。AngⅡ水平与 NF-κB 活性，AngⅡ水平与 ROS 水平均呈显著正相关关系（P ＜0．01）。表明NAC 剂量依赖性改善 COPD 病理变化，推测其可能是通过降低 AngⅡ水平，从而减少 ROS 的产生，进而抑制 NF-κB 活化过程而起效。
위관찰 N-을선반광안산（NAC）항양화작용대만성조새성폐질병（COPD）모형대서혈관활성물질적영향，장100지청길급 Wistar 대서수궤분위대조조、모형조、NAC 저제량조、중제량、고제량5조，매조20지。모형조채용기관주입지다당（LPS）화향연염독적복합방법조모。재모형조기출상，저제량조、중제량、고제량조분별안26．32、52．64、105．28 mg/kg NAC 관위，련속2주。검측병이철（MDA）、초양화물기화매（SOD）、혈관긴장소Ⅱ（AngⅡ）、활성양（ROS）、내피소（ET）、일양화담（NO）급폐조직핵전록인자κB （NF-κB）적변화，분석 AngⅡ여 ROS、NF-κB 적상관성。결과현시，여대조조비교，모형조 AngⅡ、ROS、ET、MDA 수평균현저승고이 NO、SOD 수평겁현저하강（P＜0．01），NF-κB 활성겁현저승고（P＜0．01）。여모형조비교，저제량조 NO 수평승고이 ET 수평현저하강（P＜0．05）；중제량조 AngⅡ、ROS、ET、MDA 수평하강혹현저하강이 NO、SOD 수평균승고（P ＜0．05혹 P ＜0．01），NF-κB 활성현저하강（P ＜0．05）；고제량조AngⅡ、ROS、ET、MDA 수평균현저하강이 NO、SOD 수평균겁현저승고（P ＜0．01），NF-κB 활성겁현저하강（P ＜0．01）。AngⅡ수평여 NF-κB 활성，AngⅡ수평여 ROS 수평균정현저정상관관계（P ＜0．01）。표명NAC 제량의뢰성개선 COPD 병리변화，추측기가능시통과강저 AngⅡ수평，종이감소 ROS 적산생，진이억제 NF-κB 활화과정이기효。
For the purpose of observing the effects of antioxidant N-acetylcysteine(NAC)on vasoactive sub-stances in rats with chronic obstructive pulmonary disease(COPD),100 Wistar rats,clear grade,were di-vided randomly into control group,model group,NAC low dose,middle dose and high dose 5 groups with 20 rats in each group.COPD model group was established with intratracheal instillation of lipopolysaccha-ride(LPS)and cigarette smoking.On the basis of mode group,the rats in low dose group,middle dose group and high dose group respectively were ig with NAC according to 26.32 mg/kg,52.64 mg/kg,105.28 mg/kg for 2 weeks.The levels of MDA,SOD,AngⅡ,ROS,ET,NO and NF-κB were examined,and s the corelation among AngⅡ,ROS and NF-κB was analyzed.Results showed that compared with the control group ,in the model group the levels of AngⅡ,ROS,ET and MDA all increased significantly(P <0.01) while the levels of both NO and SOD decreased significantly(P < 0.01),NF-κB activity increased(P <0.01).Compared with the model group,in the low dose group,the level of NO increased (P <0.05)while the level of ET decreased(P <0.05);in the middle dose group ,the levels of AngⅡ,ROS,ET and MDA all decreased while the levels of both NO and SOD increased(P <0.05 or P <0.01),NF-κB activity de-creased(P <0.05);in high dose group the levels of AngⅡ,ROS,ET and MDA all decreased significantly while the levels of both NO and SOD increased significantly(P <0.01),NF-κB activity decreased signifi-cantly (P <0.01).Meantime,the corelation between the level of AngⅡ ,NF-κB activity and the level of ROS had a significant corelation(P <0.01).So NAC could improve pathological changes of COPD dose-de-pendently and It was supposed to take an effect by reducing the level of AngⅡ,then decreasing the level of ROS to inhibit the activated process of NF-κB.