CAJ | 학술논문

为深入开展羊口疮病毒特性研究与羊口疮的防控，通过细胞分离培养、B2L 基因序列测定及分析等试验，对从山东某羊场分离的一株羊口疮病毒分离株 SD1201进行病毒学及分子生物学特性研究。结果表明，该分离株能够引起绵羊睾丸细胞、Vero E6细胞、MDCK 细胞发生不同程度的病变，绵羊睾丸细胞病变最为明显，在18 h 内迅速发生细胞病变，病毒 TCTD50浓度为10-4．5/mL。通过 PCR 扩增分离株 B2L基因特定片段并克隆测序，分析结果表明，该分离株与3株羊口疮病毒中国分离株亲缘关系最近，同源性为98．0％～98．2％。
위심입개전양구창병독특성연구여양구창적방공，통과세포분리배양、B2L 기인서렬측정급분석등시험，대종산동모양장분리적일주양구창병독분리주 SD1201진행병독학급분자생물학특성연구。결과표명，해분리주능구인기면양고환세포、Vero E6세포、MDCK 세포발생불동정도적병변，면양고환세포병변최위명현，재18 h 내신속발생세포병변，병독 TCTD50농도위10-4．5/mL。통과 PCR 확증분리주 B2L기인특정편단병극륭측서，분석결과표명，해분리주여3주양구창병독중국분리주친연관계최근，동원성위98．0％～98．2％。
The biological characteristics of an isolate Orf virus isolated from a goat farm in Shandong pro-vence were investigated by cell culture,PCR amplification and B2L gene sequence analysis.The results in-dicated that the isolate SD1201 was able to propagated in sheep testicular cells,Vero E6,MDCK cells and caused cytopathogenic effect in varying degrees.The infected sheep testicular cells showed most obvious CPE 18 h after inoculation,and the TCTD50 was 10 -4.5/mL.The B2L gene was amplified by PCR and the sequence analysis results indicated that the homology of nucleotide sequence was between 98.0% and 98.2% as compared with three strains isolated from China.