CAJ | 학술논문

以猪瘟病毒（CSFV）E2蛋白为研究对象，通过扩增 CSFV C 株 E2基因，亚克隆至原核表达载体 pGEX-6P-1，构建重组原核表达质粒 pGEX-6P-1-E2，转化至宿主菌 Rostta（DE3）后进行诱导表达。SDS-PAGE 检测表明与目的蛋白大小一致，其分子质量大小为67．0 ku，并且目的蛋白以包涵体形式存在。Western blot 表明，重组蛋白能与 CSFV 兔化高免血清反应并且能被 GST 标签单抗所识别，表明该融合蛋白正确表达，具有良好的抗原性，为 CSFV 抗体检测试剂盒的研究奠定了基础。
이저온병독（CSFV）E2단백위연구대상，통과확증 CSFV C 주 E2기인，아극륭지원핵표체재체 pGEX-6P-1，구건중조원핵표체질립 pGEX-6P-1-E2，전화지숙주균 Rostta（DE3）후진행유도표체。SDS-PAGE 검측표명여목적단백대소일치，기분자질량대소위67．0 ku，병차목적단백이포함체형식존재。Western blot 표명，중조단백능여 CSFV 토화고면혈청반응병차능피 GST 표첨단항소식별，표명해융합단백정학표체，구유량호적항원성，위 CSFV 항체검측시제합적연구전정료기출。
The E2 protein of CSFV was as the object and E2 gene of CSFV C strain was amplified and then sub-cloned into prokaryotic expression plasmid pGEX-6P-1 vector.The recombinant plasmid named pGEX-6P-1-E2 was transformed into E.coli Rosetta (DE3)and induced by IPTG.A specific expression band with a molecular weight 67.0 ku was detected by SDS-PAGE,and the expressed product was in inclusion body.Western blot indicated that the expressed protein can react with the rabbit antibody to CSFV and can be recognized by the specific monoclonal antibody of GST.The result suggested that the recombinant protein was correctly expressed and has a good antigenicity.This study provides substantial base for re-search of the CSFV antibody detection kit.