CAJ | 학술논문

在分析不同美洲型猪繁殖与呼吸综合征病毒（PRRSV）全基因组基础上，设计一对能扩增2种PRRSV 毒株 NSP2基因序列的特异性共有引物。RT-PCR 结果显示，变异株和经典株扩增片段分别为342 bp 和432 bp。结果表明，该方法具有快速、特异、敏感、重复性好等特点，可同时鉴别检测美洲型 PRRSV 变异毒株与经典毒株。临床应用于16份样品检测，PRRSV 总体检出率为37．5％，高致病性特征变异株占总毒株的83．3％。
재분석불동미주형저번식여호흡종합정병독（PRRSV）전기인조기출상，설계일대능확증2충PRRSV 독주 NSP2기인서렬적특이성공유인물。RT-PCR 결과현시，변이주화경전주확증편단분별위342 bp 화432 bp。결과표명，해방법구유쾌속、특이、민감、중복성호등특점，가동시감별검측미주형 PRRSV 변이독주여경전독주。림상응용우16빈양품검측，PRRSV 총체검출솔위37．5％，고치병성특정변이주점총독주적83．3％。
In order to analyze whole genome of the different American genotype porcine reproductive and re-spiratory syndrome virus(PRRSV),a pair of specific primer were designed to amplify two kinds of PRRSV strain NSP2 genes.RT-PCR test results indicated that the amplified fragments of mutant and classical strains were 342 bp and 432 bp.The method was rapid,specific,sensitive,repeatabe,and can be used to i-dentify mutant and classical strains of American genotype PRRSV at the same time.The clinical application in the detection of 16 samples showed that the positive rate of PRRSV was 37.5%,highly pathogenic char-acteristics of variant strains accounted for 83.3% of the total strains.