CAJ | 학술논문

目的：探讨半乳糖凝集素3（Galectin-3）、基质金属蛋白酶（MMP）-2在人脑胶质瘤细胞中的表达及二者在胶质瘤发生及恶性进展中的作用。方法采用免疫组化法检测5例正常脑组织及40例不同级别胶质瘤标本中 Galectin-3、MMP-2蛋白的表达情况，显微镜下根据瘤细胞中 Galectin-3、MMP-2阳性细胞数判断其表达，阳性细胞计数以阳性细胞所占总细胞数百分比的阳性指数（ LI）来表示。结果正常脑组织中 Galectin-3、MMP-2蛋白的表达均为阴性。在脑胶质瘤标本中 Galectin-3主要表达于肿瘤细胞的胞质及胞膜，Galectin-3在Ⅰ、Ⅱ级胶质瘤标本中阳性表达率为39.13％（9/23），LI 值为（5.65±3.47）％；在Ⅲ、Ⅳ级胶质瘤标本中阳性表达率为76.47％（13/17），LI 值为（27.88±22.13）％，Ⅰ、Ⅱ级与Ⅲ、Ⅳ级间阳性表达率和 LI 值比较差异有统计学意义（χ2=4.101、t =4.105，P 均＜0.05）。在脑胶质瘤中 MMP-2蛋白主要表达于瘤细胞及血管基底膜内皮细胞的胞质，MMP-2在Ⅰ、Ⅱ级胶质瘤标本中阳性表达率为39.13％（9/23），LI 值为（5.91±4.78）％，在Ⅲ、Ⅳ级胶质瘤标本中阳性表达率为88.24％（15/17），LI 值为（30.06±22.94）％，Ⅰ、Ⅱ级与Ⅲ、Ⅳ级间阳性表达率和 LI 值比较差异有统计学意义（χ2=7.882、t =4.271，P 均＜0.05）。经直线相关分析，Galectin-3与 MMP-2的阳性细胞表达率呈正相关（r =0.800，P ＜0.05）。结论 Galectin-3、MMP-2蛋白的表达在Ⅰ、Ⅱ级胶质瘤组明显低于Ⅲ、Ⅳ级组，与胶质瘤的恶性程度及进展呈正相关，更有利于对人脑胶质瘤的恶性生物学行为进行评价和判断。
목적：탐토반유당응집소3（Galectin-3）、기질금속단백매（MMP）-2재인뇌효질류세포중적표체급이자재효질류발생급악성진전중적작용。방법채용면역조화법검측5례정상뇌조직급40례불동급별효질류표본중 Galectin-3、MMP-2단백적표체정황，현미경하근거류세포중 Galectin-3、MMP-2양성세포수판단기표체，양성세포계수이양성세포소점총세포수백분비적양성지수（ LI）래표시。결과정상뇌조직중 Galectin-3、MMP-2단백적표체균위음성。재뇌효질류표본중 Galectin-3주요표체우종류세포적포질급포막，Galectin-3재Ⅰ、Ⅱ급효질류표본중양성표체솔위39.13％（9/23），LI 치위（5.65±3.47）％；재Ⅲ、Ⅳ급효질류표본중양성표체솔위76.47％（13/17），LI 치위（27.88±22.13）％，Ⅰ、Ⅱ급여Ⅲ、Ⅳ급간양성표체솔화 LI 치비교차이유통계학의의（χ2=4.101、t =4.105，P 균＜0.05）。재뇌효질류중 MMP-2단백주요표체우류세포급혈관기저막내피세포적포질，MMP-2재Ⅰ、Ⅱ급효질류표본중양성표체솔위39.13％（9/23），LI 치위（5.91±4.78）％，재Ⅲ、Ⅳ급효질류표본중양성표체솔위88.24％（15/17），LI 치위（30.06±22.94）％，Ⅰ、Ⅱ급여Ⅲ、Ⅳ급간양성표체솔화 LI 치비교차이유통계학의의（χ2=7.882、t =4.271，P 균＜0.05）。경직선상관분석，Galectin-3여 MMP-2적양성세포표체솔정정상관（r =0.800，P ＜0.05）。결론 Galectin-3、MMP-2단백적표체재Ⅰ、Ⅱ급효질류조명현저우Ⅲ、Ⅳ급조，여효질류적악성정도급진전정정상관，경유리우대인뇌효질류적악성생물학행위진행평개화판단。
Objective To investigate the significance of galactose galectin-3(Galectin-3)and matrix metalloproteinase-2(MMP-2)expression in gliomas patients and thire role in process of gliomas' malignancy development. Methods Immunohistochemistry was used to detect Galectin-3 and MMP-2 protein expression in 5 normal brain tissue and 40 patients with different grade gliomas. According to positive cells number of Galectin-3,MMP-2 in tumor cells under a microscope,to determine the expression,and the positive index(LI)which came from the percentage of the positive cell number out of the total cell number was to expressed the number of positive cell. Results Galectin-3 and MMP-2 protein expression in normal brain tissue were negative. In glioma tissues,Galectin-3 was mainly expressed in the cytoplasm and membrane of tumor cells. In 23 glioma tissue withⅠor Ⅱ grade,9 cases(39. 13% )was positive and the LI values was(5. 65 ± 3. 47)% in terms of Galectin-3 expression. In Ⅲ,Ⅳ grade glioma specimens,the positive rate of Galectin-3 expression was 76. 47%(13 / 17), and LI value was(27. 88 ± 22. 13)% . The difference of Galectin-3 expression and LI value were significant between specimens with Ⅰ,Ⅱ grade and Ⅲ,Ⅳ significant( χ2 = 4. 101,t = 4. 105;P < 0. 05). In human gliomas,MMP-2 expression protein was mainly expressed in tumor cells and vascular basement membrane of the endothelial cell cytoplasm. In 23 glioma tissue with Ⅰor Ⅱ grade,9 cases(39. 13% )was positive and the LI values was(5. 91 ± 4. 78)% in terms of MMP-2 expression. In Ⅲ,Ⅳ grade glioma specimens,the positive rate of MMP-2 expression was 88. 24%(15 / 17),and LI value was(30. 06 ± 22. 94)% . The difference of MMP-2 expression was significant between specimens with Ⅰ,Ⅱ grade or Ⅲ,Ⅳ grade( χ2 = 7. 882,t = 4. 271;P< 0. 05). The linear correlation analysis showed that there was positively correlation between Galectin-3 and MMP-2 positive cells(r = 0. 800,P < 0. 05). Conclusion Galectin-3 and MMP-2 protein expression in Ⅰ,Ⅱgrade gliomas is significantly lower than those inⅢ,Ⅳ grade glioma,and they are positively related with the progress of malignant gliomas. Galectin-3 and MMP-2 protein can be used to evaluate or judge the malignant stage of human brain glioma.