热带病与寄生虫学
熱帶病與寄生蟲學
열대병여기생충학
TROPICAL DISEASES AND PARASITOLOGY
2014年
2期
67-69,72
,共4页
卢萍%汪伟%曲国立%戴建荣%梁幼生
盧萍%汪偉%麯國立%戴建榮%樑幼生
로평%왕위%곡국립%대건영%량유생
日本血吸虫%吡喹酮%青蒿素%双氢青蒿素%青蒿琥酯%蒿甲醚%交叉抗药性
日本血吸蟲%吡喹酮%青蒿素%雙氫青蒿素%青蒿琥酯%蒿甲醚%交扠抗藥性
일본혈흡충%필규동%청호소%쌍경청호소%청호호지%호갑미%교차항약성
Schistosoma japonicum%Praziquantel%Artemisinin%Dihydroartemisinin%Artesunate%Artemether%Cross-resistance
目的:观察3种青蒿素衍生物双氢青蒿素、青蒿琥酯和蒿甲醚对日本血吸虫吡喹酮抗性株童虫的体内作用效果。方法以经11轮亚治疗剂量吡喹酮筛选的日本血吸虫为吡喹酮抗性株,以未暴露于吡喹酮的日本血吸虫作为吡喹酮敏感株,收集2虫株尾蚴感染小鼠,以300 mg/kg双氢青蒿素、青蒿琥酯和蒿甲醚对感染后7~8 d童虫分别进行2次灌服用药(总剂量600 mg/kg),所有小鼠于感染后45 d解剖,收集小鼠体内成虫并计数,计算减虫率和减雌率。结果300 mg/kg双氢青蒿素、蒿甲醚和青蒿琥酯2日疗法(总剂量600 mg/kg)对日本血吸虫吡喹酮敏感株7~8 d童虫的减虫率为69.8%~71.0%,减雌率为75.4%~79.8%;对日本血吸虫吡喹酮抗性株7~8 d童虫的减虫率为64.6%~66.1%,减雌率为69.3%~71.1%,差异均无统计学意义(均P>0.05)。结论日本血吸虫吡喹酮抗性株对青蒿素类衍生物双氢青蒿素、青蒿琥酯和蒿甲醚依然敏感,青蒿素衍生物与吡喹酮在日本血吸虫中不存在交叉抗药性。
目的:觀察3種青蒿素衍生物雙氫青蒿素、青蒿琥酯和蒿甲醚對日本血吸蟲吡喹酮抗性株童蟲的體內作用效果。方法以經11輪亞治療劑量吡喹酮篩選的日本血吸蟲為吡喹酮抗性株,以未暴露于吡喹酮的日本血吸蟲作為吡喹酮敏感株,收集2蟲株尾蚴感染小鼠,以300 mg/kg雙氫青蒿素、青蒿琥酯和蒿甲醚對感染後7~8 d童蟲分彆進行2次灌服用藥(總劑量600 mg/kg),所有小鼠于感染後45 d解剖,收集小鼠體內成蟲併計數,計算減蟲率和減雌率。結果300 mg/kg雙氫青蒿素、蒿甲醚和青蒿琥酯2日療法(總劑量600 mg/kg)對日本血吸蟲吡喹酮敏感株7~8 d童蟲的減蟲率為69.8%~71.0%,減雌率為75.4%~79.8%;對日本血吸蟲吡喹酮抗性株7~8 d童蟲的減蟲率為64.6%~66.1%,減雌率為69.3%~71.1%,差異均無統計學意義(均P>0.05)。結論日本血吸蟲吡喹酮抗性株對青蒿素類衍生物雙氫青蒿素、青蒿琥酯和蒿甲醚依然敏感,青蒿素衍生物與吡喹酮在日本血吸蟲中不存在交扠抗藥性。
목적:관찰3충청호소연생물쌍경청호소、청호호지화호갑미대일본혈흡충필규동항성주동충적체내작용효과。방법이경11륜아치료제량필규동사선적일본혈흡충위필규동항성주,이미폭로우필규동적일본혈흡충작위필규동민감주,수집2충주미유감염소서,이300 mg/kg쌍경청호소、청호호지화호갑미대감염후7~8 d동충분별진행2차관복용약(총제량600 mg/kg),소유소서우감염후45 d해부,수집소서체내성충병계수,계산감충솔화감자솔。결과300 mg/kg쌍경청호소、호갑미화청호호지2일요법(총제량600 mg/kg)대일본혈흡충필규동민감주7~8 d동충적감충솔위69.8%~71.0%,감자솔위75.4%~79.8%;대일본혈흡충필규동항성주7~8 d동충적감충솔위64.6%~66.1%,감자솔위69.3%~71.1%,차이균무통계학의의(균P>0.05)。결론일본혈흡충필규동항성주대청호소류연생물쌍경청호소、청호호지화호갑미의연민감,청호소연생물여필규동재일본혈흡충중불존재교차항약성。
Objective To evaluate the in-vivo efficacy of three artemisinin derivatives artemether, artesunate and dihydroartemisinin against schistosomulum of praziquantel-resistant Schistosoma japonicum,. Methods The S. japonicum subjected to subcurative dose of praziquantel for 11 rounds was served as a praziquantel-resistant strain, while the field-derived strain without exposure to praziquantel was served as a praziquantel-susceptible strain. The mice infected with the two strains of cercaria were treated with artemether, artesunate and dihydroartemisinin at a single dose of 300 mg/kg by gavage in days 7 and 8 post-infection, respectively. All mice were dissected 45 days post-infection, and the adult worms were collected to estimate worm and female worm burden reductions caused by drug treatment. Results Administration of artemether, artesunate and dihydroartemisinin at a single dose of 300 mg/kg given on days 7 and 8 post-infection achieved total worm burden reductions of 69.8%-71.0%and female worm burden reductions of 75.4%-79.8%in mice infected with praziquantel-susceptible strain, whereas the same treatments reduced total worm burdens of 64.6%-66.1% and female worm burdens of 69.3%-71.1% in mice infected with praziquantel-resistant strain. There were no significant differences between the two strains. Conclusion Praziquantel-resistant S. japonicum remains sensitive to the three major artemisinin derivatives artemether, artesunate and dihydroartemisinin, and no cross-resistance is observed between praziquantel and artemisinin in S. japonicum.