CAJ | 학술논문

为了解鸡毒支原体（Mycoplasmagallisepticum, MG）F弱毒疫苗株的细胞免疫特性，分别以活菌浓度为109（A组）、106（B组）ccu/mL的MG F株及生理盐水（C组）点眼接种SPF鸡，采用流式细胞技术及T淋巴细胞增殖试验对免疫前后淋巴细胞亚类Th/T、Tc/T、Th/Tc及刺激指数（SI）的动态变化规律进行研究。结果显示免疫后A、B组的Th/T、Tc/T、SI明显升高，其中Th/T于d5、d7，Tc/T、SI于d7、d14，A组显著高于B组（P<0.05）。研究结果表明，免疫MG F株可较好的提高鸡的细胞免疫，且MG F株活菌浓度与接种鸡外周血Th/T、Tc/T、SI呈正相关。
위료해계독지원체（Mycoplasmagallisepticum, MG）F약독역묘주적세포면역특성，분별이활균농도위109（A조）、106（B조）ccu/mL적MG F주급생리염수（C조）점안접충SPF계，채용류식세포기술급T림파세포증식시험대면역전후림파세포아류Th/T、Tc/T、Th/Tc급자격지수（SI）적동태변화규률진행연구。결과현시면역후A、B조적Th/T、Tc/T、SI명현승고，기중Th/T우d5、d7，Tc/T、SI우d7、d14，A조현저고우B조（P<0.05）。연구결과표명，면역MG F주가교호적제고계적세포면역，차MG F주활균농도여접충계외주혈Th/T、Tc/T、SI정정상관。
To evaluate the effect of Mycoplasma gallisepticum (MG) F vaccine on cellular immunity, SPF chickens were inoculated with 109 ccu/mL MG (A group), 106 ccu/mL MG (B group) and saline (C group), respectively. In addition, the change of Th/T, Tc/T, Th/Tc and SI were analyzed by Flow cytometry and lymphocytes proliferation test. The results showed that Th/T, Tc/T, Th/Tc and SI of A and B group increased significantly. Both Th/T on 5 and 7 days post vaccination (dpv) and Tc/T, SI on 7 and 14 dpv of A group were significantly higher than B group (P<0.05). These findings indicated that MG F vaccine was able to enhance the cellular immune response and Th/T, Tc/T and SI in peripheral blood mononuclear cells (PBMCs) were positively correlated with the inoculation close of MG F vaccine.