CAJ | 학술논문

维甲酸诱导基因-I（retinoic acid inducible gene-I，RIG-I）是最近几年发现的一种细胞内模式识别受体，能够特异性的识别并结合病毒RNA，进而发挥抗病毒效应。RIG-I在进化中相对保守，但已有报道和我们的前期研究显示：家禽中鸭和鹅体内均有RIG-I受体，但鸡却缺乏RIG-I受体。为了研究这种天然缺失对鸡是否有不利影响，本研究利用PiggyBac转座系统将鹅源RIG-I转染入鸡胚成纤维细胞系DF-1，两次亚克隆后获得单个克隆，以荧光、RT-PCR及Western blot进行鉴定，最终获得稳定表达鹅RIG-I的DF-1细胞系，为进一步研究鸡的先天性免疫机制和鹅RIG-I的功能打下基础。
유갑산유도기인-I（retinoic acid inducible gene-I，RIG-I）시최근궤년발현적일충세포내모식식별수체，능구특이성적식별병결합병독RNA，진이발휘항병독효응。RIG-I재진화중상대보수，단이유보도화아문적전기연구현시：가금중압화아체내균유RIG-I수체，단계각결핍RIG-I수체。위료연구저충천연결실대계시부유불리영향，본연구이용PiggyBac전좌계통장아원RIG-I전염입계배성섬유세포계DF-1，량차아극륭후획득단개극륭，이형광、RT-PCR급Western blot진행감정，최종획득은정표체아RIG-I적DF-1세포계，위진일보연구계적선천성면역궤제화아RIG-I적공능타하기출。
Retinoic acid inducible gene-I (RIG-I) is a family member of cytoplasmic pattern recognition receptors (PRRs), which acts as a sensor for RNA virus and plays an important role in antiviral innate immunity. RIG-I is evolutionally conserved among most species. However, several reports and our previous studies have identified that receptors for RIG-I exist in both ducks and geese, but not in chickens. To investigate whether this natural loss of RIG-I plays a negative role in chicken, goose RIG-I was transfected into chicken embryo fibroblast cell line DF-1 through PiggyBac transposon system. Monoclonal cells were obtained from twice sub-cloned cultures. DF-1 cells stably expressing goose RIG-I were identified by detection of fluorescence, RT-PCR and Western blot. The present study has laid the foundation for future research on mechanism of chicken innate immunity and function of goose RIG-I.