CAJ | 학술논문

研究表明，辐照致弱血吸虫尾蚴疫苗能够诱导宿主产生较高的免疫保护作用，是所有血吸虫候选疫苗中保护力最强的，且辐照致弱尾蚴在宿主体内不能发育成熟，不会对宿主造成严重的病理损害。单性感染的血吸虫同样不能在宿主体内发育成熟，其发育状态停留在童虫阶段，也不会对宿主造成严重的病理损害，在童虫状态下对宿主持续进行的免疫刺激，是否也能诱导宿主对继发血吸虫病产生较高的免疫保护作用呢？为此，本研究通过让宿主携带单性血吸虫雄虫的带虫免疫方式来检测其对继发血吸虫病的影响。选用雄性血吸虫尾蚴感染BALB/c小鼠（100条/只），于单性血吸虫尾蚴攻击后第5、7 w用吡喹酮进行治疗（300 mg/kg），第9 w进行雌雄血吸虫混合尾蚴攻击实验动物（40条/只），第15 w剖杀实验动物，收集宿主荷虫、宿主肝卵负荷、肝卵孵化、宿主肝单卵肉芽肿体积数据，并检测宿主血清特异性抗体变化趋势。结果表明单性带虫免疫可以引起宿主肝虫卵孵化率及组织病理损伤显著降低，对宿主有一定的免疫保护效果。
연구표명，복조치약혈흡충미유역묘능구유도숙주산생교고적면역보호작용，시소유혈흡충후선역묘중보호력최강적，차복조치약미유재숙주체내불능발육성숙，불회대숙주조성엄중적병리손해。단성감염적혈흡충동양불능재숙주체내발육성숙，기발육상태정류재동충계단，야불회대숙주조성엄중적병리손해，재동충상태하대숙주지속진행적면역자격，시부야능유도숙주대계발혈흡충병산생교고적면역보호작용니？위차，본연구통과양숙주휴대단성혈흡충웅충적대충면역방식래검측기대계발혈흡충병적영향。선용웅성혈흡충미유감염BALB/c소서（100조/지），우단성혈흡충미유공격후제5、7 w용필규동진행치료（300 mg/kg），제9 w진행자웅혈흡충혼합미유공격실험동물（40조/지），제15 w부살실험동물，수집숙주하충、숙주간란부하、간란부화、숙주간단란육아종체적수거，병검측숙주혈청특이성항체변화추세。결과표명단성대충면역가이인기숙주간충란부화솔급조직병리손상현저강저，대숙주유일정적면역보호효과。
Previous studies have shown that irradiated schistosome cercariae can induce higher immunoprotection. In the hosts, the radiation attenuated cercariae can neither mature nor cause serious pathological damage. Single-sex schistosoma perform similar behaviors but induce uninterrupted immune stimulation. Whether or not single-sex schistosoma can induce immune protection in hosts is unknown. To explore this problem, we first infected male BALB/c mice with single-sex male schistosoma and then examined its impact on the secondary schistosomiasis. BALB/c mice were randomly divided into two groups. Each mouse in test group was infected with 100 male cercariae and untreated mice used as controls. At 5 and 7 weeks post-infection, all mice in two groups were treated with praziquantel (300 mg/kg). Then at 9 weeks post-infection, all mice were infected with 40 sex-mixed cercariae. At 15 weeks post-infection, all mice were sacrificed and liver tissues and blood samples were collected for detection of eggs, pathological changes and seroconversion. The results showed that single-sex male worm caused significant reduction in eggs hatching rate and pathological damages in livers, indicating a certain immunoprotective effect in hosts.