CAJ | 학술논문

为了探讨肌醇对哲罗鲑(Hucho taimen)生长性能、体成分及消化酶活性的影响，以鱼粉、明胶和酪蛋白为蛋白源配制肌醇含量为99.8(不添加肌醇)、199.8、299.8、499.8、699.8、899.8和5099.8 mg/kg的7种饲料，分别投喂7个处理组，每组3个重复，每重复30尾鱼，进行为期56 d的饲养实验。结果表明，投喂肌醇含量为499.8 mg/kg饲料组的增重率最大，且与添加量低于299.8 mg/kg的3个饲料组差异显著(P<0.05)。饲料系数在499.8 mg/kg饲料组最低，在5099.8 mg/kg饲料组最高，两组间差异显著(P<0.05)。肌醇含量为499.8~899.8 mg/kg饲料组的特定生长率显著高于99.8~299.8 mg/kg及5099.8 mg/kg饲料组(P<0.05)。肌肉中水分、粗脂肪和粗蛋白各组间差异不显著(P>0.05)，肝淀粉酶、幽门盲囊脂肪酶活性各组间差异不显著(P>0.05)。肠道蛋白酶活性在肌醇含量为199.8~499.8 mg/kg饲料组较高，显著高于其他组(P<0.05)，脂肪酶活性在299.8~899.8 mg/kg饲料组高于99.8 mg/kg饲料组(P<0.05)。由此得出，饲料中适量添加肌醇可提高哲罗鲑的生长性能及消化酶活性；肌醇含量过低时哲罗鲑增重率、消化酶活性均较低；肌醇含量过高时哲罗鲑特定增长率、消化酶活性较低，饲料系数较高；以增重率为指标由折线回归模型分析得出，肌醇的最适含量为536.6 mg/kg饲料。
위료탐토기순대철라해(Hucho taimen)생장성능、체성분급소화매활성적영향，이어분、명효화락단백위단백원배제기순함량위99.8(불첨가기순)、199.8、299.8、499.8、699.8、899.8화5099.8 mg/kg적7충사료，분별투위7개처리조，매조3개중복，매중복30미어，진행위기56 d적사양실험。결과표명，투위기순함량위499.8 mg/kg사료조적증중솔최대，차여첨가량저우299.8 mg/kg적3개사료조차이현저(P<0.05)。사료계수재499.8 mg/kg사료조최저，재5099.8 mg/kg사료조최고，량조간차이현저(P<0.05)。기순함량위499.8~899.8 mg/kg사료조적특정생장솔현저고우99.8~299.8 mg/kg급5099.8 mg/kg사료조(P<0.05)。기육중수분、조지방화조단백각조간차이불현저(P>0.05)，간정분매、유문맹낭지방매활성각조간차이불현저(P>0.05)。장도단백매활성재기순함량위199.8~499.8 mg/kg사료조교고，현저고우기타조(P<0.05)，지방매활성재299.8~899.8 mg/kg사료조고우99.8 mg/kg사료조(P<0.05)。유차득출，사료중괄량첨가기순가제고철라해적생장성능급소화매활성；기순함량과저시철라해증중솔、소화매활성균교저；기순함량과고시철라해특정증장솔、소화매활성교저，사료계수교고；이증중솔위지표유절선회귀모형분석득출，기순적최괄함량위536.6 mg/kg사료。
Hucho taimen is a fast growing, easily domesticated, carnivorous fish that is valued for its taste and nutri-tional value. This species has been domesticated and bred successfully in more than a dozen provinces in China, in-cluding Heilongjiang, Liaoning, Shandong, and Xinjiang. Despite prior research, the nutrient requirements of H. taimen are poorly understood. We evaluated the effects of dietary myo-inositol (MI) levels on body composition, growth per-formance, and digestive enzyme activity in H. taimen. We formulated seven semipurified basal diets using fish meal, gelatin, and casein as the protein source. The MI content of the diets was 99.8, 199.8, 299.8, 499.8, 799.8, 899.8, or 5 099.8 mg MI/kg diet. Each diet was fed to triplicate groups of 30 fish (mean initial weight:2.83±0.44 g) for 56 d. The weight gain rate was highest in fish fed a diet with 499.8 mg MI/kg and was significantly higher (P<0.05) than that in fish fed diets with≤299.8 mg MI/kg. Weight gain rate was lowest in the group fed a diet with 99.8 mg MI/kg. Feed conversion ratio was lowest in H. taimen fed a diet with 499.8 mg MI/kg, and was significantly (P<0.05) lower than in H. taimen fed a diet with 299.8 mg MI/kg or 5 099.8 mg MI/kg. The feed conversion ratio was highest in fish fed a diet with 5 099.8 mg MI/kg. The specific growth rate was significantly higher (P<0.05) in fish fed 499.8-899.8 mg MI/kg than fish fed diets with≤200 or 5 099.8 mg MI/kg. Supplementation of dietary myo-inositol did not affect moisture, crude lipid, crude protein, or amylase activity in the liver and lipase activity in the pyloric caeca (P>0.05). Fish fed a diet with 499.8 mg MI/kg had the highest (P<0.05) protease activity in the liver, followed those fed by 699.8 mg MI/kg. Lipase activity increased initially and then decreased as the level of dietary MI increased. Lipase activity in the liver of H. taimen fed 299.8 mg MI/kg was significantly (P<0.05) higher than in those fed 99.8 mg MI/kg. Protease activity was significantly higher (P<0.05) in the intestine of fish fed diets with 199.8-499.8 mg MI/kg than those fed the other diets. Lipase activity was significantly higher in the intestine of fish fed 299.8-899.8 mg MI/kg than fish fed 99.8 mg MI/kg. Amylase was significantly higher (P<0.05) in the intestine of fish fed 499.8 mg MI/kg than in the remaining groups. In conclusion, supplementation of diets with moderate concentrations of myo-inositol resulted in better growth perform-ance and digestive enzyme activity in H. taimen. Feeding diets with lower MI concentrations was associated with lower weight gain, specific growth rate, crude lipid levels, and digestive enzyme activity. Feeding diets with excessive MI was associated with reduced growth performance, digestive enzyme activity and feed conversion efficiency. Based on weight gain rate using broken-line regression, the weight gain rate in H. taimen was maximized by feeding 536.6 mg MI/kg diet. Our results provide insight into the dietary MI requirements for H. taimen.