CAJ | 학술논문

本研究旨在探讨二甲基-β-丙酸噻亭(DMPT)对哲罗鱼(Hucho taimen)生长性能、肌肉成分和蛋白质代谢的影响。选取1800尾初始体质量为(4.20±0.02) g的健康幼鱼,随机分为6组,设1个对照组,5个处理组,每组3个重复,每重复100尾。各处理组分别在基础饲料中添加0.02%、0.05%、0.1%、0.2%和0.5%的DMPT,对照组投喂基础饲料。室内流水饲养周期为56 d。结果表明,饲料中DMPT添加量为0.2%时,哲罗鱼增重率和摄食率达最大值；以DMPT添加量和增重率进行二次曲线回归分析,得出DMPT最适添加量为0.24%。DMPT添加量为0.05%时,血清总蛋白、白蛋白和球蛋白水平显著提高(P<0.05)；添加0.2%DMPT时,血清总蛋白、球蛋白水平和谷草转氨酶水平显著提高(P<0.05)；添加水平0.02%~0.5%时,哲罗鱼肝和肌肉蛋白质水平显著升高(P<0.05),胃、肠道蛋白酶活性显著升高(P<0.05)；添加水平0.05%时,脏体指数显著降低(P<0.05)；各处理组肌肉脂肪和灰分含量均与对照组差异不显著(P>0.05),各组间血清谷丙转氨酶、肌肉氨基酸组成及含量未呈显著差异(P>0.05)。综上可见,饲料中添加0.2%DMPT可明显提高哲罗鱼生长性能,添加0.02%~0.5%DMPT可增加哲罗鱼肌肉和肝蛋白储存。
본연구지재탐토이갑기-β-병산새정(DMPT)대철라어(Hucho taimen)생장성능、기육성분화단백질대사적영향。선취1800미초시체질량위(4.20±0.02) g적건강유어,수궤분위6조,설1개대조조,5개처리조,매조3개중복,매중복100미。각처리조분별재기출사료중첨가0.02%、0.05%、0.1%、0.2%화0.5%적DMPT,대조조투위기출사료。실내류수사양주기위56 d。결과표명,사료중DMPT첨가량위0.2%시,철라어증중솔화섭식솔체최대치；이DMPT첨가량화증중솔진행이차곡선회귀분석,득출DMPT최괄첨가량위0.24%。DMPT첨가량위0.05%시,혈청총단백、백단백화구단백수평현저제고(P<0.05)；첨가0.2%DMPT시,혈청총단백、구단백수평화곡초전안매수평현저제고(P<0.05)；첨가수평0.02%~0.5%시,철라어간화기육단백질수평현저승고(P<0.05),위、장도단백매활성현저승고(P<0.05)；첨가수평0.05%시,장체지수현저강저(P<0.05)；각처리조기육지방화회분함량균여대조조차이불현저(P>0.05),각조간혈청곡병전안매、기육안기산조성급함량미정현저차이(P>0.05)。종상가견,사료중첨가0.2%DMPT가명현제고철라어생장성능,첨가0.02%~0.5%DMPT가증가철라어기육화간단백저존。
We evaluated the effects of dimethyl-β-propiothetin (DMPT) on growth, proximate muscle composition, and protein metabolism in Hucho taimen. Juvenile fish (n=1 800, average initial body weight:4.20±0.02 g) were cultured in a flow through system. The fish were randomly divided into six triplicate groups of 100 fish and fed diets containing different levels of DMPT (0, 0.25%, 0.5%, 0.75%, and 1.0%) for 56 d. Weight gain rate was highest at 0.2%DMPT supplementation (P<0.05). The optimum supplementation was 0.24% based on a polynomial regression analysis be-tween dietary DMPT level and weight gain rate. Serum total protein, albumin, and globulin increased significantly with 0.05% DMPT supplementation (P<0.05) and serum total protein, globulin and serum aspartate aminotransferase in-creased significantly with 0.2%DMPT supplementation (P<0.05). Meanwhile, liver and muscle protein levels and pro-tease activity in the digestive track increased significantly as dietary DMPT supplementation increased between 0.02%and 0.5%(P<0.05). The liver somatic index increased significantly with 0.05%DMPT supplementation. DMPT sup-plementation had no effect on muscle crude lipid, ash, amino acid profile, or serum alanine transaminase (P>0.05). Our results suggest that growth performance and protein levels were improved by dietary DMPT supplementation in taimen and 0.02%-0.5%DMPT supplementation can increase the protein storage in muscle and liver.