检验检疫学刊
檢驗檢疫學刊
검험검역학간
INSPECTION AND QUARANTINE SCIENCE
2014年
3期
65-67
,共3页
程宝艳%刘长坤%黄金华%邵建明%胡晓苗%赵瑞宏%张丹俊
程寶豔%劉長坤%黃金華%邵建明%鬍曉苗%趙瑞宏%張丹俊
정보염%류장곤%황금화%소건명%호효묘%조서굉%장단준
禽白血病病毒%p27抗原%抗体%感染状况
禽白血病病毒%p27抗原%抗體%感染狀況
금백혈병병독%p27항원%항체%감염상황
Avian Leukosis Virus%p27 Antigen%Antibody%Infection Situations
[目的]了解禽白血病病毒在安徽辖区进口鸡群中的感染情况。[方法]随机采集1日龄胎粪、不同日龄段泄殖腔棉拭子检测禽白血病病毒群特异性p27抗原、采集血清检测ALV-AB及J亚型抗体，收集种蛋检测蛋清p27抗原。[结果]祖代蛋鸡1日龄胎粪和200日龄泄殖腔棉拭子p27抗原的阳性率分别为18.2%、45%、4.5%、15%；种蛋蛋清p27抗原全为阴性，父母代蛋鸡180日龄泄殖腔棉拭子和种蛋清p27抗原的阳性率分别为21.9%、10%；祖代和父母代蛋鸡ALV-J抗体全为阴性，ALV-AB抗体阳性率分别为9%、10%、28.1%。祖代肉鸡泄殖腔棉拭子p27抗原阳性率分别为5%、6.7%、3.3%，种蛋蛋清p27抗原全为阴性；哈伯德鸡群ALV-J抗体为阴性，AA鸡群ALV-J抗体阳性率为20%；两个品种不同日龄肉鸡ALV-AB抗体的阳性率分别为32.5%、63.3%、80%。[结论]安徽进口鸡群中ALV-J仅AA鸡群有20%的感染率，ALV-AB的感染率分别为9%、10%、28.1%、32.5%、63.3%、80%。
[目的]瞭解禽白血病病毒在安徽轄區進口鷄群中的感染情況。[方法]隨機採集1日齡胎糞、不同日齡段洩殖腔棉拭子檢測禽白血病病毒群特異性p27抗原、採集血清檢測ALV-AB及J亞型抗體，收集種蛋檢測蛋清p27抗原。[結果]祖代蛋鷄1日齡胎糞和200日齡洩殖腔棉拭子p27抗原的暘性率分彆為18.2%、45%、4.5%、15%；種蛋蛋清p27抗原全為陰性，父母代蛋鷄180日齡洩殖腔棉拭子和種蛋清p27抗原的暘性率分彆為21.9%、10%；祖代和父母代蛋鷄ALV-J抗體全為陰性，ALV-AB抗體暘性率分彆為9%、10%、28.1%。祖代肉鷄洩殖腔棉拭子p27抗原暘性率分彆為5%、6.7%、3.3%，種蛋蛋清p27抗原全為陰性；哈伯德鷄群ALV-J抗體為陰性，AA鷄群ALV-J抗體暘性率為20%；兩箇品種不同日齡肉鷄ALV-AB抗體的暘性率分彆為32.5%、63.3%、80%。[結論]安徽進口鷄群中ALV-J僅AA鷄群有20%的感染率，ALV-AB的感染率分彆為9%、10%、28.1%、32.5%、63.3%、80%。
[목적]료해금백혈병병독재안휘할구진구계군중적감염정황。[방법]수궤채집1일령태분、불동일령단설식강면식자검측금백혈병병독군특이성p27항원、채집혈청검측ALV-AB급J아형항체，수집충단검측단청p27항원。[결과]조대단계1일령태분화200일령설식강면식자p27항원적양성솔분별위18.2%、45%、4.5%、15%；충단단청p27항원전위음성，부모대단계180일령설식강면식자화충단청p27항원적양성솔분별위21.9%、10%；조대화부모대단계ALV-J항체전위음성，ALV-AB항체양성솔분별위9%、10%、28.1%。조대육계설식강면식자p27항원양성솔분별위5%、6.7%、3.3%，충단단청p27항원전위음성；합백덕계군ALV-J항체위음성，AA계군ALV-J항체양성솔위20%；량개품충불동일령육계ALV-AB항체적양성솔분별위32.5%、63.3%、80%。[결론]안휘진구계군중ALV-J부AA계군유20%적감염솔，ALV-AB적감염솔분별위9%、10%、28.1%、32.5%、63.3%、80%。
The research aimed to investigate the infection situations of avian leukosis virus(ALV) in import chickens of Anhui Province. The meconium of 1 day age, cloacal swabs and serum of different ages were randomly collected in these farms. They were tested for p27 antigen, ALV-AB and ALV-J subtype antibody. Collecting eggs after they began laying, p27 antigen was detected in egg white. The positive rate of p27 antigen for progenitor layer 1 day old meconium and 200 day old cloacal swab were 18.2%, 45%, 4.5%, 15%; all hatching eggs were negative for p27 detection. The positive rate of p27 antigen for parent layer 180 day old cloacal swabs and egg white were 21.9%, 10%;All the grandparent and parent layer ALV-J antibody were negative, the positive rate of ALV-AB antibody were 9%, 10%, 28.1%respectively. The positive rate of p27 antigen of chicken cloacal swab progenitor were respectively 5%, 6.7%, 3.3%, while the p27 antigen of egg white was negative. Hubbard chickens were negative to ALV-J antibody, whereas the positive rate of AA chickens was 20%. The positive rate of ALV-AB antibody for two varieties of different day old broiler were 32.5%, 63.3%, 80%. There was just 20%AA chickens infected with ALV-J in import chickens of Anhui Province, while ALV-AB infection rates were 9%, 10%, 28.1%, 32.5%, 63.3%, 80%.
