浙江科技学院学报
浙江科技學院學報
절강과기학원학보
JOURNAL OF ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
2014年
3期
172-176
,共5页
周天琼%俞保彬%倪燕红%周一峰%毛建卫
週天瓊%俞保彬%倪燕紅%週一峰%毛建衛
주천경%유보빈%예연홍%주일봉%모건위
转移因子胶囊%多肽%核糖%含量测定
轉移因子膠囊%多肽%覈糖%含量測定
전이인자효낭%다태%핵당%함량측정
transfer factor capsules%polypeptide%ribose%content determination
研究了转移因子胶囊的多肽与核糖含量测定方法。分别对福林酚法和紫外-可见分光光度法测定转移因子胶囊多肽和核糖含量的方法进行了考察。福林酚法对牛血清白蛋白在30~300μg/m L范围内线性关系良好(r=0.9990),平均加样回收率为99.77%,RSD为1.31%;紫外-可见分光光度法对D-核糖2~10μg/mL范围内线性关系良好( r=0.9997),平均加样回收率99.99%,RSD为1.02%。以上分析方法简便、快速、准确,可作为转移因子胶囊多肽与核糖含量分析的方法。
研究瞭轉移因子膠囊的多肽與覈糖含量測定方法。分彆對福林酚法和紫外-可見分光光度法測定轉移因子膠囊多肽和覈糖含量的方法進行瞭攷察。福林酚法對牛血清白蛋白在30~300μg/m L範圍內線性關繫良好(r=0.9990),平均加樣迴收率為99.77%,RSD為1.31%;紫外-可見分光光度法對D-覈糖2~10μg/mL範圍內線性關繫良好( r=0.9997),平均加樣迴收率99.99%,RSD為1.02%。以上分析方法簡便、快速、準確,可作為轉移因子膠囊多肽與覈糖含量分析的方法。
연구료전이인자효낭적다태여핵당함량측정방법。분별대복림분법화자외-가견분광광도법측정전이인자효낭다태화핵당함량적방법진행료고찰。복림분법대우혈청백단백재30~300μg/m L범위내선성관계량호(r=0.9990),평균가양회수솔위99.77%,RSD위1.31%;자외-가견분광광도법대D-핵당2~10μg/mL범위내선성관계량호( r=0.9997),평균가양회수솔99.99%,RSD위1.02%。이상분석방법간편、쾌속、준학,가작위전이인자효낭다태여핵당함량분석적방법。
The analytical methods for polypeptide and ribose in transfer factor capsule were studied .The feasibility of Folin phenol method and UV-vis spectrophotometry method for polypeptide and ribose were investigated .The linear range of bovine serum albumin is 30-300μg/mL(r= 0 .999 0) ,and the average recovery of bovine serum albumin is 99 .77% (RSD 1.31% ) .The linear range of D-ribose is 2-10 μg/mL(r=0 .999 7) ,and the average recovery of D-ribose is 99 .99% (RSD 1 .02% ) . The methods reported in this paper are efficient , accurate and suitable for determination for polypeptide and ribose in transfer factor capsules .