内蒙古民族大学学报(自然科学版)
內矇古民族大學學報(自然科學版)
내몽고민족대학학보(자연과학판)
JOURNAL OF INNER MONGOLIA UNIVERSITY FOR NATIONALITIES(NATURAL SCIENCES)
2014年
3期
277-279
,共3页
周风琴%赵玉英%张良%姚立伟%王海燕
週風琴%趙玉英%張良%姚立偉%王海燕
주풍금%조옥영%장량%요립위%왕해연
哈斯-哈图古日-15%没食子酸%HPLC
哈斯-哈圖古日-15%沒食子痠%HPLC
합사-합도고일-15%몰식자산%HPLC
HPLC%Haas-Hatuguri-15%Gallic Acid
建立用HPLC法测定蒙药哈斯-哈图古日-15中没食子酸的方法.色谱柱为:Hypcrsil C18 ODS(4.6mm×250mm,5μL),流动相为乙腈-水-磷酸(体积比为5:95:0.02),流速为0.8 mL·min-1,检测波长为273nm,柱温为30℃.没食子酸在(0.40~1.2)mg·mL-1范围内线性关系良好(y=9E+06x-127588,r=0.9999),平均回收率94.95%(RSD=2.24%).该方法可作为蒙药及蒙药材的没食子酸的含量测定方法.
建立用HPLC法測定矇藥哈斯-哈圖古日-15中沒食子痠的方法.色譜柱為:Hypcrsil C18 ODS(4.6mm×250mm,5μL),流動相為乙腈-水-燐痠(體積比為5:95:0.02),流速為0.8 mL·min-1,檢測波長為273nm,柱溫為30℃.沒食子痠在(0.40~1.2)mg·mL-1範圍內線性關繫良好(y=9E+06x-127588,r=0.9999),平均迴收率94.95%(RSD=2.24%).該方法可作為矇藥及矇藥材的沒食子痠的含量測定方法.
건립용HPLC법측정몽약합사-합도고일-15중몰식자산적방법.색보주위:Hypcrsil C18 ODS(4.6mm×250mm,5μL),류동상위을정-수-린산(체적비위5:95:0.02),류속위0.8 mL·min-1,검측파장위273nm,주온위30℃.몰식자산재(0.40~1.2)mg·mL-1범위내선성관계량호(y=9E+06x-127588,r=0.9999),평균회수솔94.95%(RSD=2.24%).해방법가작위몽약급몽약재적몰식자산적함량측정방법.
To establish a HPLC method for determination the content of Gallic Acid in Haas-Hatuguri-15. The high performance liquid chromatography(HPLC)separation was performend on Hypcrsil C18 ODS(4.6mm×250mm,5μL). The mobile phase consisted of acetonitrile-water-phosphate (The volume ratio of 5:95:0.02 ). The flow rate was 0.8mL·min-1 , the detection wavelength was 273nm, the column temperation was 30℃. Gallic Acid showed a good lin-ear relationship(y=9E+06x-127588,r=0.9999)in the range of 0.40~1.20mg·mL-1 , the average recovery rate was 94.95%, RSD=2.24%. This method can be used as the Gallic Acid in Mongolian medicine and Mongolian medicine de-termination method.