CAJ | 학술논문

为探明嘧菌酯在牧草中的安全性，对嘧菌酯在北京、江苏南京、河南郑州3个试验点牧草和土壤中的残留消解动态和最终残留规律进行了研究。牧草和土壤样品经乙酸乙酯提取，氨基柱净化，液相色谱-质谱检测器检测，外标法峰面积定量。在0．01，0．10和1．00 mg/kg 3个添加水平，嘧菌酯在牧草中的添加回收率为86．5％～97．4％，相对标准偏差为6．1％～10．3％；在土壤中平均回收率为82．4％～107．2％，相对标准偏差为2．3％～5．7％；嘧菌酯在牧草和土壤中的最低检出限均为0．01 mg/kg。嘧菌酯在北京、江苏南京和河南郑州3个试验点牧草中的半衰期为2．9～3．4 d，在土壤中的半衰期为3．2～3．7 d。参照食品法典委员会（Codex Alimentarius Com-mission，CAC）制定的饲料和干秸秆中嘧菌酯的最大残留限量标准（15 mg/kg），按推荐施药剂量和次数施用嘧菌酯21 d后，所采收的牧草是安全的。
위탐명밀균지재목초중적안전성，대밀균지재북경、강소남경、하남정주3개시험점목초화토양중적잔류소해동태화최종잔류규률진행료연구。목초화토양양품경을산을지제취，안기주정화，액상색보-질보검측기검측，외표법봉면적정량。재0．01，0．10화1．00 mg/kg 3개첨가수평，밀균지재목초중적첨가회수솔위86．5％～97．4％，상대표준편차위6．1％～10．3％；재토양중평균회수솔위82．4％～107．2％，상대표준편차위2．3％～5．7％；밀균지재목초화토양중적최저검출한균위0．01 mg/kg。밀균지재북경、강소남경화하남정주3개시험점목초중적반쇠기위2．9～3．4 d，재토양중적반쇠기위3．2～3．7 d。삼조식품법전위원회（Codex Alimentarius Com-mission，CAC）제정적사료화간갈간중밀균지적최대잔류한량표준（15 mg/kg），안추천시약제량화차수시용밀균지21 d후，소채수적목초시안전적。
To determine the safety of azoxystrobin in forages,residue degradation and final residue levels of azoxystrobin in forages and soils at three experimental sites (Beijing,Nanjing of Jiangsu province and Zheng-zhou of Henan province)were determined by HPLC-MS.Residues of azoxystrobin in forage and soil were ex-tracted by ethyl acetate,cleaned in a NH2-column and detected by HPLC-MS.External standards were em-ployed for quantitative analysis.The method was validated at three levels of fortification (0.01,0.10 and 1.00 mg/kg)in forages and soils.The recoveries of azoxystrobin in forages were 86.5%-97.4%,with RSD of 6.1%-10.3%,and the recoveries in soils were 82.4%-107.2%,with RSD of 2.3%-5.7%.The limit of detection of azoxystrobin in forages and soils was 0.01 mg/kg.The half-lives of residues of azoxystrobin in for-ages at the three sites were 2.9-3.4 days,and the half-lives in soils were 3.2-3.7 days.Twenty one days af-ter treatment,azoxystrobin residues in forages were safe according to the recommended dosage and times based on the CAC’s of azoxystrobin in forages (1 5 mg/kg).