草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2014年
3期
333-342
,共10页
陈伟%张苗苗%宋阳阳%陈建纲%张德罡
陳偉%張苗苗%宋暘暘%陳建綱%張德罡
진위%장묘묘%송양양%진건강%장덕강
重金属离子%高羊茅%多年生黑麦草%叶绿素荧光特性%根系形态
重金屬離子%高羊茅%多年生黑麥草%葉綠素熒光特性%根繫形態
중금속리자%고양모%다년생흑맥초%협록소형광특성%근계형태
heavy metal%tall fescue%perennial ryegrass%fluorescence characteristics%root morphology
以高羊茅和多年生黑麦草为试验材料,分别设置Cd2+、Cu2+、Zn2+、Pb2+不同浓度处理,研究4种重金属离子对其荧光特性及根系形态的影响。结果表明,高羊茅在4种重金属离子处理下,其最大量子产量(Fv/Fm )值均低于对照,说明高羊茅在受到重金属离子胁迫后,其 PSⅡ活性受到伤害;多年生黑麦草在 Cd2+不同浓度处理下,其Fv/Fm值均略高于对照,但差异不显著,说明Cd2+胁迫对多年生黑麦草的PSⅡ系统的活性影响不大;但是 Cu2+和Zn2+浓度分别达到500 mg/kg及以上时,对多年生黑麦草Fv/Fm 值影响较大,均显著低于对照;同时,多年生黑麦草还表现出低浓度Zn2+促进PSⅡ系统活性的作用,但随着浓度升高,表现出对PSⅡ系统越来越强的抑制作用;在4种重金属离子不同浓度处理下,2种草坪草的光化学淬灭系数(qP)和电子传递速率(ETR)值均低于对照,说明其光合活性和电子传递效率均受到影响;经过4种重金属离子处理后,除部分离子低浓度处理外,高羊茅总根长、根表面积、总分叉数和总根尖数均显著低于对照,并且有随着离子处理浓度升高而逐渐降低的趋势;多年生黑麦草根系则表现出对低浓度重金属离子抗性较强,对高浓度重离子抗性较差,并且也有随浓度升高而不断降低的趋势。
以高羊茅和多年生黑麥草為試驗材料,分彆設置Cd2+、Cu2+、Zn2+、Pb2+不同濃度處理,研究4種重金屬離子對其熒光特性及根繫形態的影響。結果錶明,高羊茅在4種重金屬離子處理下,其最大量子產量(Fv/Fm )值均低于對照,說明高羊茅在受到重金屬離子脅迫後,其 PSⅡ活性受到傷害;多年生黑麥草在 Cd2+不同濃度處理下,其Fv/Fm值均略高于對照,但差異不顯著,說明Cd2+脅迫對多年生黑麥草的PSⅡ繫統的活性影響不大;但是 Cu2+和Zn2+濃度分彆達到500 mg/kg及以上時,對多年生黑麥草Fv/Fm 值影響較大,均顯著低于對照;同時,多年生黑麥草還錶現齣低濃度Zn2+促進PSⅡ繫統活性的作用,但隨著濃度升高,錶現齣對PSⅡ繫統越來越彊的抑製作用;在4種重金屬離子不同濃度處理下,2種草坪草的光化學淬滅繫數(qP)和電子傳遞速率(ETR)值均低于對照,說明其光閤活性和電子傳遞效率均受到影響;經過4種重金屬離子處理後,除部分離子低濃度處理外,高羊茅總根長、根錶麵積、總分扠數和總根尖數均顯著低于對照,併且有隨著離子處理濃度升高而逐漸降低的趨勢;多年生黑麥草根繫則錶現齣對低濃度重金屬離子抗性較彊,對高濃度重離子抗性較差,併且也有隨濃度升高而不斷降低的趨勢。
이고양모화다년생흑맥초위시험재료,분별설치Cd2+、Cu2+、Zn2+、Pb2+불동농도처리,연구4충중금속리자대기형광특성급근계형태적영향。결과표명,고양모재4충중금속리자처리하,기최대양자산량(Fv/Fm )치균저우대조,설명고양모재수도중금속리자협박후,기 PSⅡ활성수도상해;다년생흑맥초재 Cd2+불동농도처리하,기Fv/Fm치균략고우대조,단차이불현저,설명Cd2+협박대다년생흑맥초적PSⅡ계통적활성영향불대;단시 Cu2+화Zn2+농도분별체도500 mg/kg급이상시,대다년생흑맥초Fv/Fm 치영향교대,균현저저우대조;동시,다년생흑맥초환표현출저농도Zn2+촉진PSⅡ계통활성적작용,단수착농도승고,표현출대PSⅡ계통월래월강적억제작용;재4충중금속리자불동농도처리하,2충초평초적광화학쉬멸계수(qP)화전자전체속솔(ETR)치균저우대조,설명기광합활성화전자전체효솔균수도영향;경과4충중금속리자처리후,제부분리자저농도처리외,고양모총근장、근표면적、총분차수화총근첨수균현저저우대조,병차유수착리자처리농도승고이축점강저적추세;다년생흑맥초근계칙표현출대저농도중금속리자항성교강,대고농도중리자항성교차,병차야유수농도승고이불단강저적추세。
The impacts of Cu2+,Zn2+,Pb2+ and Cd2+ stresses on fluorescence characteristics and root morphol-ogy of tall fescue (Festuca elata)and perennial ryegrass (Poa pratensis)were studied.The Fv/Fm of tall fes-cue under Cu2+,Zn2+,Pb2+ and Cd2+ stresses were lower than that in the controls suggesting that the activity of PSⅡ was weakened.However,it was quite different for perennial ryegrass.The Fv/Fm under Cd2+ stress was slightly higher than that in the controls indicating that the impact of Cd2+ was not significant.However,it was significantly reduced when the concentrations of Cu2+ and Zn2+ reached 500 mg/kg or more.Meanwhile, the lower concentrations of Zn2+ resulted in an improving effect on the PSⅡ system.The qP and ETR of both species under the stresses of 4 heavy metals were lower than those in the controls suggesting that the photosyn-thetic activity and electron transformation efficiency were inhibited.Except at low concentrations,the root length,root area,numbers of root forks and tips of tall fescue were significantly lower than those in the con-trols when under stress from four heavy metals:there was a decreasing trend with increasing stress concentra-tion.Perennial ryegrass showed a strong resistance to the stresses when heavy metals were at low concentra-tions and vice versa.