CAJ | 학술논문

马铃薯坏疽病、炭疽病和枯萎病是甘肃省马铃薯贮藏期最主要的病害，为了获得马铃薯贮藏期病害防治的生防菌株，利用对峙培养法从东祁连山高寒草地线叶嵩草内生细菌中筛选获得高抗菌株263XY1，其对马铃薯枯萎病菌、马铃薯炭疽病菌和马铃薯坏疽病菌的抑菌率分别达到67．17％，86．17％和70．89％；该菌株在含有色氨酸和不含色氨酸的金氏培养基中分泌的 IAA分别为3．31和1．52 mg/L，在 PKO 含无机磷培养基中的有效磷增量为38．94 mg/L，具有固氮能力。经16S rDNA 序列分析，其与死谷芽孢杆菌（Bacillus vallismortis，登录号：JQ547633．1）同源性达99％，初步鉴定为死谷芽孢杆菌。
마령서배저병、탄저병화고위병시감숙성마령서저장기최주요적병해，위료획득마령서저장기병해방치적생방균주，이용대치배양법종동기련산고한초지선협숭초내생세균중사선획득고항균주263XY1，기대마령서고위병균、마령서탄저병균화마령서배저병균적억균솔분별체도67．17％，86．17％화70．89％；해균주재함유색안산화불함색안산적금씨배양기중분비적 IAA분별위3．31화1．52 mg/L，재 PKO 함무궤린배양기중적유효린증량위38．94 mg/L，구유고담능력。경16S rDNA 서렬분석，기여사곡아포간균（Bacillus vallismortis，등록호：JQ547633．1）동원성체99％，초보감정위사곡아포간균。
Phoma foveata,Colletotrichum coccodes and Fusarium avenaceum are 3 species of potato storage pathogens.The highly resistant bacterial strain 263XY1 tested against potato storage diseases was screened from endophytic bacteria isolated from Kobreasia capillifolia under alpine grassland in the Eastern Qilian Mountains using the confront culture method.The inhibition rates of 263XY1 against F.avenaceum,C.coc-codes and P.foveata were 67.17%,86.17% and 70.89%,respectively.The concentration of IAA secreted by 263XY1 was 3.31 mg/L in the King medium with Trp,and 1.52 mg/L without Trp.The increment of availa-ble P was 38.94 mg/L obtained by dissolving inorganic P (phosphate of calcium)and bacterial strain 263XY1 possessed the ability to fix nitrogen.The 16S rDNA sequence analysis indicated that 263XY1 shared 99% ho-mology with Bacillusvallismortis (GenBank accession No.JQ547633.1),so 263XY1 was identified as B.val-lismortis.