草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2014年
3期
182-190
,共9页
刘成%陈晓德%吴明%贺丽%叶小齐%范庭兴
劉成%陳曉德%吳明%賀麗%葉小齊%範庭興
류성%진효덕%오명%하려%협소제%범정흥
化感%芦苇%加拿大一枝黄花%抗氧化酶%根系活力
化感%蘆葦%加拿大一枝黃花%抗氧化酶%根繫活力
화감%호위%가나대일지황화%항양화매%근계활력
allelopathy%Phragmites communis%Solidago canadensis%antioxidant enzyme%root activity
采用砂培法研究了不同浓度(0,0.025,0.050和0.100 g DW/mL)芦苇叶水浸提液对入侵植物加拿大一枝黄花的化感效应。结果表明,在试验过程中(0~45 d),随芦苇浸提液浓度升高,加拿大一枝黄花基径和株高的累积生长量显著下降,死亡数随浓度升高明显上升;叶绿素含量随处理时间持续降低,且浓度越高叶绿素含量越低,而类胡萝卜素含量则呈先升高后降低之势;低浓度时,过氧化物酶(POD)和超氧化物歧化酶(SOD)活性随处理时间持续上升,但当浓度继续升高,则二者均呈先升高后降低之势;过氧化氢酶活性(CAT)和丙二醛(MDA)含量随芦苇叶浸提液浓度升高而上升,且二者随处理时间呈持续升高之势;可溶性蛋白含量和根系活力随处理浓度升高而降低,且该趋势随处理时间延长而加剧。可见,随浸提液浓度升高和处理时间延长,芦苇对加拿大一枝黄花化感胁迫效应增强。
採用砂培法研究瞭不同濃度(0,0.025,0.050和0.100 g DW/mL)蘆葦葉水浸提液對入侵植物加拿大一枝黃花的化感效應。結果錶明,在試驗過程中(0~45 d),隨蘆葦浸提液濃度升高,加拿大一枝黃花基徑和株高的纍積生長量顯著下降,死亡數隨濃度升高明顯上升;葉綠素含量隨處理時間持續降低,且濃度越高葉綠素含量越低,而類鬍蘿蔔素含量則呈先升高後降低之勢;低濃度時,過氧化物酶(POD)和超氧化物歧化酶(SOD)活性隨處理時間持續上升,但噹濃度繼續升高,則二者均呈先升高後降低之勢;過氧化氫酶活性(CAT)和丙二醛(MDA)含量隨蘆葦葉浸提液濃度升高而上升,且二者隨處理時間呈持續升高之勢;可溶性蛋白含量和根繫活力隨處理濃度升高而降低,且該趨勢隨處理時間延長而加劇。可見,隨浸提液濃度升高和處理時間延長,蘆葦對加拿大一枝黃花化感脅迫效應增彊。
채용사배법연구료불동농도(0,0.025,0.050화0.100 g DW/mL)호위협수침제액대입침식물가나대일지황화적화감효응。결과표명,재시험과정중(0~45 d),수호위침제액농도승고,가나대일지황화기경화주고적루적생장량현저하강,사망수수농도승고명현상승;협록소함량수처리시간지속강저,차농도월고협록소함량월저,이류호라복소함량칙정선승고후강저지세;저농도시,과양화물매(POD)화초양화물기화매(SOD)활성수처리시간지속상승,단당농도계속승고,칙이자균정선승고후강저지세;과양화경매활성(CAT)화병이철(MDA)함량수호위협침제액농도승고이상승,차이자수처리시간정지속승고지세;가용성단백함량화근계활력수처리농도승고이강저,차해추세수처리시간연장이가극。가견,수침제액농도승고화처리시간연장,호위대가나대일지황화화감협박효응증강。
A sandy culture experiment was conducted to study the allelopathic effects of different concentrations (0,0.025 g DW/mL,0.05 g DW/mL and 0.1 g DW/mL)of aqueous extract from Phragmites communis leaves on the growth and physiobiochemical characteristics of the invasive plant species Solidago canadensis. During the test (0-45 d),cumulative growth of basal diameter and height of S.canadensis was significantly reduced with stronger extract concentrations while the mortality significantly increased.Chlorophyll content continued to decrease with time,and the higher the extract concentration the lower the chlorophyll content, while carotenoid content initially increased but then decreased.When treated at a low concentration (0.025 g DW/mL),the activities of peroxidase (POD)and superoxide dismutase (SOD)increased as processing time was prolonged,but when the concentration continued to rise,they both increased initially but then decreased. Catalase activity (CAT)and malondialdehyde (MDA)content increased when the concentration of extract in-creased,and both increased with processing time.Soluble protein content and root activity decreased at higher concentrations,and the trend was exacerbated with prolonged processing.The results indicate that allelopathic effects of P.communis on S.canadensis became stronger with increased concentration and processing time.