草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2014年
3期
160-166
,共7页
孟亚雄%王世红%汪军成%徐先良%赖勇%司二静%马小乐%李葆春%杨轲%王化俊
孟亞雄%王世紅%汪軍成%徐先良%賴勇%司二靜%馬小樂%李葆春%楊軻%王化俊
맹아웅%왕세홍%왕군성%서선량%뢰용%사이정%마소악%리보춘%양가%왕화준
CoCl2%NaCl胁迫%内源激素%抗氧化酶%大麦
CoCl2%NaCl脅迫%內源激素%抗氧化酶%大麥
CoCl2%NaCl협박%내원격소%항양화매%대맥
CoCl2%NaCl stress%endogenous hormone%antioxidant enzymes%Hordeumvulgare
为了明确钴对 NaCl胁迫的缓解作用,本研究就CoCl2对不同浓度 NaCl胁迫下大麦种子的萌发、幼苗生长及内源激素与相关生理指标的影响进行了探讨。结果表明,随着 NaCl胁迫程度的加重,大麦种子发芽势、发芽率显著降低,芽与根的生长受到显著抑制。幼苗脱落酸(ABA)与丙二醛(MDA)大量积累,抗氧化酶活性提高。加入0.1 mmol/L CoCl2显著提高了 NaCl胁迫下大麦种子的发芽势与发芽率,促进了芽与根的生长,抑制了幼苗 ABA的积累,同时提高了抗氧化酶活性,降低了 MDA 的含量,且在较严重 NaCl 胁迫下作用更明显。CoCl2提高了NaCl胁迫下幼苗(IAA+GA3)/ABA的值,减缓了活性氧(ROS)的积累与膜脂过氧化进程,对种子萌发及幼苗生长起到了促进作用,在一定程度上提高了大麦幼苗对 NaCl胁迫的适应能力。
為瞭明確鈷對 NaCl脅迫的緩解作用,本研究就CoCl2對不同濃度 NaCl脅迫下大麥種子的萌髮、幼苗生長及內源激素與相關生理指標的影響進行瞭探討。結果錶明,隨著 NaCl脅迫程度的加重,大麥種子髮芽勢、髮芽率顯著降低,芽與根的生長受到顯著抑製。幼苗脫落痠(ABA)與丙二醛(MDA)大量積纍,抗氧化酶活性提高。加入0.1 mmol/L CoCl2顯著提高瞭 NaCl脅迫下大麥種子的髮芽勢與髮芽率,促進瞭芽與根的生長,抑製瞭幼苗 ABA的積纍,同時提高瞭抗氧化酶活性,降低瞭 MDA 的含量,且在較嚴重 NaCl 脅迫下作用更明顯。CoCl2提高瞭NaCl脅迫下幼苗(IAA+GA3)/ABA的值,減緩瞭活性氧(ROS)的積纍與膜脂過氧化進程,對種子萌髮及幼苗生長起到瞭促進作用,在一定程度上提高瞭大麥幼苗對 NaCl脅迫的適應能力。
위료명학고대 NaCl협박적완해작용,본연구취CoCl2대불동농도 NaCl협박하대맥충자적맹발、유묘생장급내원격소여상관생리지표적영향진행료탐토。결과표명,수착 NaCl협박정도적가중,대맥충자발아세、발아솔현저강저,아여근적생장수도현저억제。유묘탈락산(ABA)여병이철(MDA)대량적루,항양화매활성제고。가입0.1 mmol/L CoCl2현저제고료 NaCl협박하대맥충자적발아세여발아솔,촉진료아여근적생장,억제료유묘 ABA적적루,동시제고료항양화매활성,강저료 MDA 적함량,차재교엄중 NaCl 협박하작용경명현。CoCl2제고료NaCl협박하유묘(IAA+GA3)/ABA적치,감완료활성양(ROS)적적루여막지과양화진정,대충자맹발급유묘생장기도료촉진작용,재일정정도상제고료대맥유묘대 NaCl협박적괄응능력。
To determine the beneficial effect of cobalt on the growth of Hordeum vulgare under NaCl stress, the effects of addition of CoCl2 on seed germination,seedling growth,endogenous hormones and some physio-logical indexes of barley seedlings under NaCl stress were studied.With the aggravation of NaCl stress,the germination vigor,germination capacity,shoot length and root length decreased considerably,while the con-tents of abscisic acid (ABA)and malondialdehyde (MDA)increased significantly,and the activities of antioxi-dant enzymes were enhanced.Under NaCl stress,germination vigor,germination capacity,shoot length and root length and the activities of antioxidant enzymes were elevated by the treatment of 0.1 mmol/L CoCl2 , compared with NaCl stress alone,and it dramatically inhibited the accumulation of MDA and ABA.This effect was more significant under the more serious NaCl stress.The results showed that the addition of CoCl2 could improve (IAA+GA3 )/ABA,enhance the ability to clear up reactive oxygen species,reduce lipid peroxidation under NaCl stress,and promote seed germination,seedling growth and adaptive capacity of barley.