CAJ | 학술논문

以1年生梭梭幼株为材料，采用盆栽试验研究不同浓度（0，50，100，200，400 mmol/L）NaCl处理对梭梭生长状况及同化枝过氧化氢（H2 O2）、丙二醛（MDA）含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）、抗坏血酸过氧化物酶（APX）活性、水势、可溶性糖和脯氨酸含量的影响。结果表明，低浓度的 NaCl处理（50 mmol/L）对梭梭的生长具有促进作用，梭梭株高和基茎粗在400 mmol/L NaCl处理下较对照显著减少，冠幅面积和同化枝、茎干重在 NaCl 浓度为≥200 mmol/L 时较对照开始显著减少，根干重在 NaCl 浓度为≥100 mmol/L时较对照呈显著减少趋势。因此，NaCl胁迫对梭梭根生长的抑制作用大于对冠幅面积，同化枝和茎干重大于对株高和基茎粗。根冠比在 NaCl 浓度≥100 mmol/L 时较对照显著减少。H2 O2和 MDA 含量在高浓度NaCl处理下较对照出现积累现象。低浓度NaCl处理下（≤100 mmol/L），梭梭同化枝SOD、POD、CAT和APX活性较对照均有所提高，它们协调一致的共同作用，有效地清除植物体内的活性氧（reactive oxygen species，ROS），高浓度的 NaCl处理下（≥200 mmol/L），CAT和POD活性较对照开始降低，抗氧化酶系统对 ROS的清除效率减弱。梭梭同化枝水势随 NaCl处理浓度升高而降低。可溶性糖和脯氨酸含量随 NaCl 浓度增加较对照先上升后降低。
이1년생사사유주위재료，채용분재시험연구불동농도（0，50，100，200，400 mmol/L）NaCl처리대사사생장상황급동화지과양화경（H2 O2）、병이철（MDA）함량、초양화물기화매（SOD）、과양화경매（CAT）、과양화물매（POD）、항배혈산과양화물매（APX）활성、수세、가용성당화포안산함량적영향。결과표명，저농도적 NaCl처리（50 mmol/L）대사사적생장구유촉진작용，사사주고화기경조재400 mmol/L NaCl처리하교대조현저감소，관폭면적화동화지、경간중재 NaCl 농도위≥200 mmol/L 시교대조개시현저감소，근간중재 NaCl 농도위≥100 mmol/L시교대조정현저감소추세。인차，NaCl협박대사사근생장적억제작용대우대관폭면적，동화지화경간중대우대주고화기경조。근관비재 NaCl 농도≥100 mmol/L 시교대조현저감소。H2 O2화 MDA 함량재고농도NaCl처리하교대조출현적루현상。저농도NaCl처리하（≤100 mmol/L），사사동화지SOD、POD、CAT화APX활성교대조균유소제고，타문협조일치적공동작용，유효지청제식물체내적활성양（reactive oxygen species，ROS），고농도적 NaCl처리하（≥200 mmol/L），CAT화POD활성교대조개시강저，항양화매계통대 ROS적청제효솔감약。사사동화지수세수 NaCl처리농도승고이강저。가용성당화포안산함량수 NaCl 농도증가교대조선상승후강저。
A pot experiment was conducted to study the influence of different concentrations (0,50,100,200 and 400 mmol/L)of NaCl on the growth,the contents of hydrogen peroxide (H2 O2 )and malondialdehyde (MDA),the activities of superoxide dismutase (SOD),catalase (CAT),peroxidase (POD)and ascorbate per-oxidase (APX),water potential,and on the contents of soluble sugar and proline in the assimilation shoots of Haloxylonammodendron.The growth of H.ammodendron was promoted at a lower NaCl concentration (50 mmol/L).Compared with the control,plant height and basal stem diameter were significantly inhibited in the 400 mmol/L NaCl treatment,while crown area,dry mass of assimilation shoot and shoot dry mass were con-siderably reduced under NaCl treatments at concentrations ≥200 mmol/L and root dry mass was considerably reduced under NaCl treatments at concentrations ≥100 mmol/L.Thus,the suppressive effect on root dry mass was greater than on crown area,dry mass of assimilation shoot and shoot dry mass,which were more af-fected than plant height and basal stem diameter.Root/shoot ratios decreased markedly under NaCl treatments at concentrations ≥100 mmol/L compared with the control.Compared with the control,there were significant accumulations of H2 O2 and MDA in the assimilation shoot of H.ammodendron at higher NaCl concentrations and the activities of SOD,POD,CAT and APX increased at low NaCl concentrations (≤100 mmol/L),sug-gesting that together they play an important role in the scavenging of reactive oxygen species.However,the activities of CAT and POD began to reduce at NaCl concentrations ≥200 mmol/L compared with the control, indicating the scavenging efficiency of the antioxidative enzyme system weakened.Water potential reduced with an increase in NaCl concentrations.The contents of soluble sugar and proline initially increased but then de-creased compared with the control.