CAJ | 학술논문

新疆塔克拉玛干沙漠中富含多种沙漠藻类，严酷的沙漠环境造就了这些沙漠微藻独特的生物学特性，但对于这些沙漠微藻的研究，相关报道较少。本研究以新疆塔克拉玛干沙漠沙样为素材，通过实验室培养，以有限稀释法和平板法进行分离，分离得到6株沙漠微藻。形态学观察后初步鉴定5株属于绿藻门（TLD2A1，TLD2B， TLD7A-2，TLD6B，TLD7B-5），1株为蓝藻门（TLD5A1）。分别比对分析了5株绿藻的18S rDNA和其中3株的5．8S rDNA-ITS序列，1株蓝藻 TLD5A1的16S rDNA 序列，并进行了系统发育分析。结果表明，TLD2A1和TLD6 B与小球藻科物种亲缘关系很近，TLD2 B和衣藻科物种亲缘关系很近。TLD7 B-5形态呈新月形，分子鉴定与环藻科物种亲缘关系较近，TLD5 A1与念珠藻科的物种聚为一支，同时与念珠藻属的椭孢念珠藻同源性高达97％。本研究确定了6株沙漠微藻的分类地位，为后续塔克拉玛干沙漠微藻的研究工作提供理论依据。
신강탑극랍마간사막중부함다충사막조류，엄혹적사막배경조취료저사사막미조독특적생물학특성，단대우저사사막미조적연구，상관보도교소。본연구이신강탑극랍마간사막사양위소재，통과실험실배양，이유한희석법화평판법진행분리，분리득도6주사막미조。형태학관찰후초보감정5주속우록조문（TLD2A1，TLD2B， TLD7A-2，TLD6B，TLD7B-5），1주위람조문（TLD5A1）。분별비대분석료5주록조적18S rDNA화기중3주적5．8S rDNA-ITS서렬，1주람조 TLD5A1적16S rDNA 서렬，병진행료계통발육분석。결과표명，TLD2A1화TLD6 B여소구조과물충친연관계흔근，TLD2 B화의조과물충친연관계흔근。TLD7 B-5형태정신월형，분자감정여배조과물충친연관계교근，TLD5 A1여념주조과적물충취위일지，동시여념주조속적타포념주조동원성고체97％。본연구학정료6주사막미조적분류지위，위후속탑극랍마간사막미조적연구공작제공이론의거。
The unique biological property of desert algae comes from the extreme environment of the Taklima-kan Desert in Xinjiang,but so far there are no related reports on the desert algae.To study the microalgae spe-cies of the Takilimakan Desert,6 desert microalgae were isolated from sand samples using 9 6-well plate limited dilution and spread plate methods.Morphologically,5 of the strains investigated were Chlorophyta (TLD2A1, TLD2B,TLD6B,TLD7A-2,TLD7B-5)while TLD5A1 was a Cyanobacterium.TLD2A1 and TLD2B showed a close relationship with Chlorellaceae,TLD2B and TLD7A-2 with Chlamydomonadaceae,and TLD7B-5 with Tetranephris brasiliensis of the Sphaeropleales.TLD5A1 and Nostoc clustered in a single clade,and the TLD5A1 strain was 97% similar to Nostocellipsosporum.This study determined the taxonomic status of the 6 strains of microalgae in the desert,providing a theoretical basis for future research of the Taklimakan Desert al-gae.