CAJ | 학술논문

目的：观察miR-19b对巨噬细胞 ABCA1表达的影响。方法在 THP-1源性巨噬细胞中转染miR-19b模拟物(mimic )和抑制物(inhibitor),荧光定量PCR检测ABCA1 mRNA,Western blot检测ABCA1蛋白水平。结果转染miR-19b mimic后,巨噬细胞ABCA1 mRNA及蛋白水平均显著下调；转染miR-19b inhibitor后ABCA1蛋白则明显上调,但ABCA1 mRNA无变化。结论 miR-19b下调巨噬细胞ABCA1的表达。
목적：관찰miR-19b대거서세포 ABCA1표체적영향。방법재 THP-1원성거서세포중전염miR-19b모의물(mimic )화억제물(inhibitor),형광정량PCR검측ABCA1 mRNA,Western blot검측ABCA1단백수평。결과전염miR-19b mimic후,거서세포ABCA1 mRNA급단백수평균현저하조；전염miR-19b inhibitor후ABCA1단백칙명현상조,단ABCA1 mRNA무변화。결론 miR-19b하조거서세포ABCA1적표체。
Objective To investigate the impact of miR-19b on the expression of macrophage ABCA1. Methods The protein and mRNA levels of ABCA1 were detected by Western blot and quantitative real time PCR ( qRT-PCR) respec-tively in THP-1 macrophage transfected with miR-19b mimic and inhibitor. Results The protein and mRNA levels of ABCA1 were obviously downregulated in THP-1 macrophage transfected with miR-19b mimic. The protein level of ABCA1 was evidently upregulated in THP-1 macrophage transfected with miR-19b inhibitor,while ABCA1 mRNA level had no sig-nificantly alternation. Conclusion miR-19b regulates the expression of macrophage ABCA1.