CAJ | 학술논문

目的：以结肠癌细胞SW480为模型,初步探讨Kiss-1基因过表达与结肠癌细胞增殖的关系。方法采用脂质体转染的方法将Kiss-1/pEGFP-N1重组质粒转染至SW480细胞,上调结肠癌细胞中Kiss-1的表达水平,经G418筛选出稳定表达株；根据细胞分为3组：阴性对照组(转染pEGFP-N1空载体)、实验组(转染pEGFP-N1-Kiss1)及空白对照组(未转染)。 Western blot检测转染后细胞中 Kiss-1表达量的变化；采用 CCK8试验分析Kiss-1对SW480细胞增殖的抑制效果。结果成功将pEGFP-N1-Kiss-1重组质粒转染至SW480结肠癌细胞, western blot检测出阴性对照组和空白对照组中显示Kiss-1低表达,实验组Kiss-1表达量增加,CCK8试验表明实验组细胞增殖率明显低于对照组(P＜0.05)。结论 Kiss-1可抑制结肠癌细胞的增殖率。
목적：이결장암세포SW480위모형,초보탐토Kiss-1기인과표체여결장암세포증식적관계。방법채용지질체전염적방법장Kiss-1/pEGFP-N1중조질립전염지SW480세포,상조결장암세포중Kiss-1적표체수평,경G418사선출은정표체주；근거세포분위3조：음성대조조(전염pEGFP-N1공재체)、실험조(전염pEGFP-N1-Kiss1)급공백대조조(미전염)。 Western blot검측전염후세포중 Kiss-1표체량적변화；채용 CCK8시험분석Kiss-1대SW480세포증식적억제효과。결과성공장pEGFP-N1-Kiss-1중조질립전염지SW480결장암세포, western blot검측출음성대조조화공백대조조중현시Kiss-1저표체,실험조Kiss-1표체량증가,CCK8시험표명실험조세포증식솔명현저우대조조(P＜0.05)。결론 Kiss-1가억제결장암세포적증식솔。
Objective To use colon cancer cell SW480 as a model,to explore effects of overexpression of Kiss-1 on proliferation of colon cancer cells. Methods Using liposome transfection,Kiss-1/pEGFP-N1 recombinant plasmid was transfected into SW480 cells,colon cancer Kiss-1 cells were upregulated,stably expressed cells were selected by G418;the cells were divided into 3 groups:negative control group ( pEGFP-N1 transfection with empty vector ) , experimental group (with pEGFP-N1-Kiss1) and control group (without transfection). Western blot detection was used to detect the expression of Kiss-1 after transfection and CCK8 test was applied to analyze the inhibitory effect of Kiss-1 on the proliferation of SW480 cells. Results pEGFP-N1-Kiss-1 recombinant plasmid was successfully transfected into the colorectal cancer cell line SW480,Western blot detected low expression of Kiss-1 in the negative control group and blank control group,while Kiss-1 in experimental group was increased;CCK8 test showed significantly lower rate of proliferation of cells in the experimental group than the control group (P<0. 05). Conclusion Kiss-1 can inhibit the proliferation of colon cancer cells rate.