中国处方药
中國處方藥
중국처방약
CHINA PRESCRIPTION DRUG
2014年
6期
44-45
,共2页
四神丸%腹泻%5-羟色胺%SP蛋白
四神汍%腹瀉%5-羥色胺%SP蛋白
사신환%복사%5-간색알%SP단백
Sishen Pill%Diarrhea%5-HT%SP protein
目的：通过观察腹泻小鼠胃肠运动和腹泻大鼠结肠中胃肠肽蛋白表达的变化，探讨四神丸对腹泻的影响。方法采用大黄泻下法复制模型，以稀便级、腹泻率、稀便率、腹泻指数、碳末推进法观测小肠碳末推进率代表胃肠运动情况，将造模成功实验大鼠分为空白对照组、模型对照组、西药对照组、四神丸组，给药后对大鼠结肠通过免疫组织化学方法检测5-羟色胺（5-HT）、P物质（SP）蛋白表达的情况，观察四神丸对其干预作用。结果经大黄泻下法复制模型后，生大黄使小鼠的胃肠运动加快，大鼠结肠中5-HT和SP蛋白表达明显升高。四神丸可以减慢腹泻小鼠胃肠运动，降低腹泻大鼠结肠中5-HT和SP蛋白表达。结论四神丸有止泻作用，可明显抑制胃肠运动，可能是通过干预5-HT、SP蛋白表达而起作用。
目的：通過觀察腹瀉小鼠胃腸運動和腹瀉大鼠結腸中胃腸肽蛋白錶達的變化，探討四神汍對腹瀉的影響。方法採用大黃瀉下法複製模型，以稀便級、腹瀉率、稀便率、腹瀉指數、碳末推進法觀測小腸碳末推進率代錶胃腸運動情況，將造模成功實驗大鼠分為空白對照組、模型對照組、西藥對照組、四神汍組，給藥後對大鼠結腸通過免疫組織化學方法檢測5-羥色胺（5-HT）、P物質（SP）蛋白錶達的情況，觀察四神汍對其榦預作用。結果經大黃瀉下法複製模型後，生大黃使小鼠的胃腸運動加快，大鼠結腸中5-HT和SP蛋白錶達明顯升高。四神汍可以減慢腹瀉小鼠胃腸運動，降低腹瀉大鼠結腸中5-HT和SP蛋白錶達。結論四神汍有止瀉作用，可明顯抑製胃腸運動，可能是通過榦預5-HT、SP蛋白錶達而起作用。
목적：통과관찰복사소서위장운동화복사대서결장중위장태단백표체적변화，탐토사신환대복사적영향。방법채용대황사하법복제모형，이희편급、복사솔、희편솔、복사지수、탄말추진법관측소장탄말추진솔대표위장운동정황，장조모성공실험대서분위공백대조조、모형대조조、서약대조조、사신환조，급약후대대서결장통과면역조직화학방법검측5-간색알（5-HT）、P물질（SP）단백표체적정황，관찰사신환대기간예작용。결과경대황사하법복제모형후，생대황사소서적위장운동가쾌，대서결장중5-HT화SP단백표체명현승고。사신환가이감만복사소서위장운동，강저복사대서결장중5-HT화SP단백표체。결론사신환유지사작용，가명현억제위장운동，가능시통과간예5-HT、SP단백표체이기작용。
Objective By animal experiment observed changes of gastrointestinal motility in mice with experimental diarrhea and the expression of gastrointestinal peptides protein in rat with experimental diarrhea, to investigate the effect of Sishen Pill on diarrhea. Methods Establish model by rhubarb purge, degree of diarrhea, the diarrhea rate, loose stools rate, diarrhea index, carbon particle propelling detection to observe the propellent rate of small intestine, represent gastrointestinal motility . Experimental rat of successful modeling were divided into four groups, blank control group, model group, western medicine control group, Sishen Pill group. After the last administration, the expression of 5-HT and the expression of SP were detected by immunohistochemical method in colon, to observe intervention effect of Sishen Pill. Results By establish model by rhubarb purge, rhubarb lead to accelerate gastrointestinal motility, the expression of 5-HT the expression of SP were enhanced in colon of rat. Sishen Pill could slow gastrointestinal motility in mice, the expression of 5-HT the expression of SP were reduced. Conclusions Sishen Pill could antidiarrheal, inhibit significantly gastrointestinal motility, intervene possibly the expression of 5-HT the expression of SP and play a role.
