中国处方药
中國處方藥
중국처방약
CHINA PRESCRIPTION DRUG
2014年
6期
41-42
,共2页
KH34%荧光%质粒构建%荧光显微镜
KH34%熒光%質粒構建%熒光顯微鏡
KH34%형광%질립구건%형광현미경
KH34%Fluorescence%Plasmid construction%Fluorescence microscopy
目的：构建KH34基因的荧光表达质粒。方法应用分子生物学手段将KH34基因克隆入phage-UBC-flag-cherry载体，构建KH34的荧光表达质粒，并以该质粒转染293T细胞，荧光显微镜下观察该质粒在细胞内的定位。结果成功构建了KH34的荧光表达质粒，并成功转染293T细胞。结论本实验成功构建了KH34的荧光表达质粒，转染后的293T细胞也具有明显的红色荧光，为后续分析KH34对细胞的功能影响奠定了基础。
目的：構建KH34基因的熒光錶達質粒。方法應用分子生物學手段將KH34基因剋隆入phage-UBC-flag-cherry載體，構建KH34的熒光錶達質粒，併以該質粒轉染293T細胞，熒光顯微鏡下觀察該質粒在細胞內的定位。結果成功構建瞭KH34的熒光錶達質粒，併成功轉染293T細胞。結論本實驗成功構建瞭KH34的熒光錶達質粒，轉染後的293T細胞也具有明顯的紅色熒光，為後續分析KH34對細胞的功能影響奠定瞭基礎。
목적：구건KH34기인적형광표체질립。방법응용분자생물학수단장KH34기인극륭입phage-UBC-flag-cherry재체，구건KH34적형광표체질립，병이해질립전염293T세포，형광현미경하관찰해질립재세포내적정위。결과성공구건료KH34적형광표체질립，병성공전염293T세포。결론본실험성공구건료KH34적형광표체질립，전염후적293T세포야구유명현적홍색형광，위후속분석KH34대세포적공능영향전정료기출。
Obiective Construction of fluorescence expression plasmid of KH34 gene. Methods Application of molecular biology methods, The KH34 gene was cloned into phage-UBC-flag-cherry vector to construct the fluorescence expression plasmid of KH34, and then the plasmid was transfected into 293T cells, observing the location of the plasmid in 293T cells under fluorescence microscope. Results The fluorescence expression plasmid of KH34 gene was constructed, and it was transfected into 293T cells. Conclusion We successfully constructed the fluorescence expression plasmid of KH34 gene, and there was visibly fluorescence in the transfected 293T cells, this laid a foundation for the subsequent analysis of KH34 on cell function.
