CAJ | 학술논문

高效液相色谱法测定非发酵豆制品中人工合成色素的含量。样品用石油醚去脂，用硫酸和钨酸钠溶液除去蛋白质，聚酰胺粉吸附色素，洗涤后解吸，纯化后测定。采用 Thermo ODS-2 HYPERSIL（150 mm ×4.6 mm ×5μm）色谱柱，流动相为0.02 mol/ L 乙酸铵-甲醇，梯度洗脱，流速为1.0 mL/ min，柱温30℃，检测波长427，485 nm，进样量20μL。结果表明，柠檬黄进样量在4~160 ng（Y ＝3079.0X -1980.4，r ＝0.9998）、日落黄进样量在7~280 ng（Y ＝2537.1X -1507.1，r ＝1.0000）范围内呈现良好的线性，柠檬黄、日落黄平均加样回收率为100.8%，100.4%。该方法能快速检测、操作简便且灵敏度高。
고효액상색보법측정비발효두제품중인공합성색소적함량。양품용석유미거지，용류산화오산납용액제거단백질，취선알분흡부색소，세조후해흡，순화후측정。채용 Thermo ODS-2 HYPERSIL（150 mm ×4.6 mm ×5μm）색보주，류동상위0.02 mol/ L 을산안-갑순，제도세탈，류속위1.0 mL/ min，주온30℃，검측파장427，485 nm，진양량20μL。결과표명，저몽황진양량재4~160 ng（Y ＝3079.0X -1980.4，r ＝0.9998）、일락황진양량재7~280 ng（Y ＝2537.1X -1507.1，r ＝1.0000）범위내정현량호적선성，저몽황、일락황평균가양회수솔위100.8%，100.4%。해방법능쾌속검측、조작간편차령민도고。
To develop a quantitative method for artificial synthetic pigment in non-fermented bean products by HPLC. Used petroleum ether degreasing method,removal of the protein in solution of sulfuric acid and sodium tungstate,polyamide adsorption pigments,determine after washing with purified analysis. The col-umn for Thermo ODS-2 HYPERSIL(150 mm × 4. 6 mm × 5 μm),the mobile phase was 0. 02 mol/ L am-monium acetate methanol,gradient elution;the flow rate was 1. 0 mL/ min;column temperature was 30 ℃;the detection wavelength:427,485 nm;sample 20 μL. Results showed that the lemon yellow in the 4 ~160 ng(Y =3 079. 0X -1 980. 4,r =0. 999 8),sunset yellow in the 7 ~ 280 ng(Y = 2 537. 1X - 1 507. 1, r = 1. 000 0)range showed a good linear,lemon yellow,sunset yellow average recovery rate of 100. 8% , 100. 4% . The rapid detection method has the advantages of simple operation,high sensitivity.