应用化工
應用化工
응용화공
APPLIED CHEMICAL INDUSTRY
2014年
6期
1097-1100,1104
,共5页
金银花%多糖%大孔吸附树脂%响应面法
金銀花%多糖%大孔吸附樹脂%響應麵法
금은화%다당%대공흡부수지%향응면법
Lonicera japonica%polysaccharide%macroporous adsorption resin%response surface analysis
采用单因素法筛选合适的树脂,依据 Box-Benhnken 的中心组合试验设计原理,设计上样量、上柱流速、上样浓度的3因素3水平响应面优化实验,得到回归方程。结果表明,金银花多糖脱色最优工艺参数为:上样量1.4 BV,上样流速1.5 BV/ h,上样浓度3 mg/ mL,在该工艺条件下,金银花多糖脱色率为91.04%,多糖保留率为81.29%,脱蛋白率为83.83%。
採用單因素法篩選閤適的樹脂,依據 Box-Benhnken 的中心組閤試驗設計原理,設計上樣量、上柱流速、上樣濃度的3因素3水平響應麵優化實驗,得到迴歸方程。結果錶明,金銀花多糖脫色最優工藝參數為:上樣量1.4 BV,上樣流速1.5 BV/ h,上樣濃度3 mg/ mL,在該工藝條件下,金銀花多糖脫色率為91.04%,多糖保留率為81.29%,脫蛋白率為83.83%。
채용단인소법사선합괄적수지,의거 Box-Benhnken 적중심조합시험설계원리,설계상양량、상주류속、상양농도적3인소3수평향응면우화실험,득도회귀방정。결과표명,금은화다당탈색최우공예삼수위:상양량1.4 BV,상양류속1.5 BV/ h,상양농도3 mg/ mL,재해공예조건하,금은화다당탈색솔위91.04%,다당보류솔위81.29%,탈단백솔위83.83%。
Screening of suitable resin by single factor analysis method,based on central composite experi-mental design principle of Box-Benhnken,response surface analysis for optimization design of three factors on the column flow rate,sample volume,sample concentration level of three,the regression equation was obtained. The result showed that the modified Lonicera japonica polysaccharide decolorization optimal process parameters were as follows:the sample amount is 1. 4 BV,elution flow rate is 1. 5 BV/ h,the con-centration of the sample is 3 mg / mL,Lonicera japonica polysaccharide decolorization rate is 91. 04% ,pol-ysaccharide retention rate is 81. 29% ,protein removal rate is 83. 83% .