CAJ | 학술논문

为确定具有较好酪氨酸酶抑制作用的酵母多肽的分子量，对酵母多肽粗制品溶液以截留相对分子量为10 KDa、5 KDa、1 KDa 的超滤膜进行超滤，采用生化酶法对不同超滤组分进行酪氨酸酶抑制活性检测。结果显示，各多肽组分的活性随相对分子量的减少而增大，活性多肽的相对分子量主要集中在1 KDa 以下。对相对分子量﹤1 KDa 的多肽组分依次以 DEAE-52阴离子交换柱层析法和 Sephadex G-15凝胶层柱析法进行分离，最后得到4个洗脱组分，分别富集各洗脱组分并进行酪氨酸酶抑制活性研究。结果表明，组分 d-1活性最佳，其 IC50值为3.53 mg/ mL。经高效液相空间排阻色谱分析，d-1组分多肽的相对分子质量主要集中在200~800 Da。
위학정구유교호락안산매억제작용적효모다태적분자량，대효모다태조제품용액이절류상대분자량위10 KDa、5 KDa、1 KDa 적초려막진행초려，채용생화매법대불동초려조분진행락안산매억제활성검측。결과현시，각다태조분적활성수상대분자량적감소이증대，활성다태적상대분자량주요집중재1 KDa 이하。대상대분자량﹤1 KDa 적다태조분의차이 DEAE-52음리자교환주층석법화 Sephadex G-15응효층주석법진행분리，최후득도4개세탈조분，분별부집각세탈조분병진행락안산매억제활성연구。결과표명，조분 d-1활성최가，기 IC50치위3.53 mg/ mL。경고효액상공간배조색보분석，d-1조분다태적상대분자질량주요집중재200~800 Da。
In order to determine the molecular weight distribution of yeast peptides with tyrosinase inhibi-ton from yeast peptides,the crude products of yeast peptides(CPYP)solution was successively ultrafil-trated with 10 KDa,5 KDa and 1 KDa ultrafiltration membrane. The tyrosinase inhibition of each fraction was evaluated in vitro. Result showed that the lower molecular-weight peptides fraction shows higher activ-ity of tyrosinase inhibition. The ultrafiltration fraction of high activity(MW ﹤ 1 KDa)was further purified using DEAE-52 anion exchange chromatography and Sephadex G-15 gel filtration chromatography. Final-ly,four fractions were obtained and their tyrosinase inhibition was evaluated respectively. The results indi-cate that fraction d-1 has higher inhibitory effect on tyrosinase activity with the IC50 of 3. 53 mg / mL. The molecular weight distribution of the fraction d-1 is ranged from 200 ~ 800 Da.