CAJ | 학술논문

目的：观察肝硬化大鼠中缝背核五羟色胺（5-hydroxytryptamine，5-HT）能神经元以及双标神经元的表达变化。方法健康雄性Wistar大鼠20只，分为模型组和对照组各10只，模型组大鼠经皮下注射CCl4结合饮用酒精14周，对照组皮下注射等剂量的生理盐水。应用5-HT免疫组织化学及5-HT复合NADPH-d免疫组织化学染色双标方法，观察中缝背核5-HT能神经元以及双标神经元的表达变化。结果与对照组相比，模型组大鼠中缝背核5-HT能神经元的数量未见明显变化，直径明显变小，模型组免疫反应阳性强度明显较对照组增强，神经元的光密度（0.54±0.04）vs（0.48±0.04）（P＜0.01）；双标神经元未见明显变化。结论肝硬化影响了大鼠中缝背核5-HT能神经元。
목적：관찰간경화대서중봉배핵오간색알（5-hydroxytryptamine，5-HT）능신경원이급쌍표신경원적표체변화。방법건강웅성Wistar대서20지，분위모형조화대조조각10지，모형조대서경피하주사CCl4결합음용주정14주，대조조피하주사등제량적생리염수。응용5-HT면역조직화학급5-HT복합NADPH-d면역조직화학염색쌍표방법，관찰중봉배핵5-HT능신경원이급쌍표신경원적표체변화。결과여대조조상비，모형조대서중봉배핵5-HT능신경원적수량미견명현변화，직경명현변소，모형조면역반응양성강도명현교대조조증강，신경원적광밀도（0.54±0.04）vs（0.48±0.04）（P＜0.01）；쌍표신경원미견명현변화。결론간경화영향료대서중봉배핵5-HT능신경원。
Objective To study the expression of 5-hydroxytryptamine( 5-HT ) and double-labeled neurons in dorsal raphe of cirrhosis rats. Methods The healthy male Wistar rats were divided into model and control groups(n=10). Within 14 weeks,model group rats were subcutaneously injected by CCl4 plus drinking ethonal. Control group rats received subcutaneously injection of sodium chloride. 5-HT immunohistochemistry and 5-HT immunohistochemistry combined with NADPH-d staining were used to detect the expression of 5-HT and double-labeled neurons in dorsal raphe of rats. Results The average diameter 5-HT neurons in dorsal raphe decreased significantly in the model group compared with that of control group. The optical density value of 5-HT neurons in dorsal raphed increased sinificantly in the mode of group(0. 54 ± 0. 04)compared with that of control group(0. 48 ± 0. 04)(P <0. 01). The quantity of 5-HT neurons and the double-labeled neurons in dorsal raphe hasd no significant difference between two groups. Conclusion Cirrhosis influenced 5-HT neurons in dorsal raphe of rats.