CAJ | 학술논문

目的：探讨体外受精-胚胎移植中人卵巢黄素化颗粒细胞端粒酶的活性与相应卵巢反应性和胚胎质量的关系。方法收集56例行体外受精-胚胎移植患者卵泡液中的黄素化颗粒细胞，并进行体外培养；根据卵泡数不同分为低反应组、中反应组和高反应组；又根据优质胚胎率不同分为低质量组和高质量组。端粒重复序列扩增酶联免疫吸附分析法测定颗粒细胞端粒酶活性，并比较低反应组、中反应组与高反应组以及低质量组与高质量组颗粒细胞端粒酶的活性。结果分离提纯的人黄素化颗粒细胞于24h内贴壁生长良好。随着患者的年龄、使用促性腺激素量的增加，端粒酶活性ΔA值逐渐降低，随着获卵数的增加端粒酶活性ΔA值逐渐上升，即端粒酶活性与年龄、促性腺激素量呈负相关，端粒酶活性与获卵数呈正相关（ P＜0.05）；低反应组与中反应组和高反应组之间差异均有统计学意义（ P＜0.01）。随着优质胚胎率的升高，颗粒细胞端粒酶活性也逐渐升高，二者呈正相关（ P＜0.01）；胚胎高质量组与低质量组颗粒细胞端粒酶活性差异有统计学意义（ P＜0.01）。结论体外培养的人卵巢黄素化颗粒细胞存在端粒酶活性的表达。黄素化颗粒细胞端粒酶活性降低与卵巢功能的下降可能有关，颗粒细胞的凋亡可能影响卵母细胞的发育潜能。
목적：탐토체외수정-배태이식중인란소황소화과립세포단립매적활성여상응란소반응성화배태질량적관계。방법수집56례행체외수정-배태이식환자란포액중적황소화과립세포，병진행체외배양；근거란포수불동분위저반응조、중반응조화고반응조；우근거우질배태솔불동분위저질량조화고질량조。단립중복서렬확증매련면역흡부분석법측정과립세포단립매활성，병비교저반응조、중반응조여고반응조이급저질량조여고질량조과립세포단립매적활성。결과분리제순적인황소화과립세포우24h내첩벽생장량호。수착환자적년령、사용촉성선격소량적증가，단립매활성ΔA치축점강저，수착획란수적증가단립매활성ΔA치축점상승，즉단립매활성여년령、촉성선격소량정부상관，단립매활성여획란수정정상관（ P＜0.05）；저반응조여중반응조화고반응조지간차이균유통계학의의（ P＜0.01）。수착우질배태솔적승고，과립세포단립매활성야축점승고，이자정정상관（ P＜0.01）；배태고질량조여저질량조과립세포단립매활성차이유통계학의의（ P＜0.01）。결론체외배양적인란소황소화과립세포존재단립매활성적표체。황소화과립세포단립매활성강저여란소공능적하강가능유관，과립세포적조망가능영향란모세포적발육잠능。
Objective To explore the relationship of telomerase activity of human luteined granulosa cells( GCs)with ovarian response and embryo quality. Methods Fity-six cases receiving IVF-ET were used to collect the data of GCs. The experiment was carried out through the following procedures:luteined GCs were isolated from follicle fluid and were cultured 24 hours soon;tests of the telomerase activity of GCs with TRAP-ELISA. Comparisons of age,number of ampules of gonadotropin ( Gn ) administered,number of retrieved oocytes,rate of high quality embryo with telomerase activity were made in different ovarian response and embryo quality groups. Results Isolated and purified GCs were cultured and adherenced well in 24 hours. There were negative correlation between telomerase activity of GCs with age,number of ampules of Gn administered and positive correlation with number of retrieved oocytes( P<0 . 05 ). Low response group had lower telomerase activity in GCs compared to medium and high response groups( P<0 . 01 ). There were positive correlation between telomerase activity of GCs with ratio of high embryo quality( P<0 . 01 ). There was significant difference of telomerase activity of GCs in different embryo quality groups( P<0 . 01 ). Conclusion Telomerase activity could be tested in human luteinized GCs after in vitro culture. It may suggest that telomerase activity decreased with the decline in ovarian function and the apoptosis of GCs may directly affect oocyte developmental potential.