中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
11期
4-4,5
,共2页
高效液相色谱法%月季花%金丝桃苷%含量测定
高效液相色譜法%月季花%金絲桃苷%含量測定
고효액상색보법%월계화%금사도감%함량측정
HPLC%rosa chinensis jacq%hyPerin%content determination
目的：建立测定月季花中金丝桃苷含量的高效液相色谱法。方法色谱柱为Agilent C18柱(100 mm ×4.6 mm，2.7μm )，流动相为乙腈-0.05%磷酸溶液（16：84），检测波长为360 nm，流速为0.5 mL/min。结果金丝桃苷进样量在0.04768~0.47680μg范围内与峰面积呈良好线性关系，平均回收率为97.22%，RSD＝0.89%( n＝9)。结论该方法快速简便，稳定可靠，专属性强。
目的：建立測定月季花中金絲桃苷含量的高效液相色譜法。方法色譜柱為Agilent C18柱(100 mm ×4.6 mm，2.7μm )，流動相為乙腈-0.05%燐痠溶液（16：84），檢測波長為360 nm，流速為0.5 mL/min。結果金絲桃苷進樣量在0.04768~0.47680μg範圍內與峰麵積呈良好線性關繫，平均迴收率為97.22%，RSD＝0.89%( n＝9)。結論該方法快速簡便，穩定可靠，專屬性彊。
목적：건립측정월계화중금사도감함량적고효액상색보법。방법색보주위Agilent C18주(100 mm ×4.6 mm，2.7μm )，류동상위을정-0.05%린산용액（16：84），검측파장위360 nm，류속위0.5 mL/min。결과금사도감진양량재0.04768~0.47680μg범위내여봉면적정량호선성관계，평균회수솔위97.22%，RSD＝0.89%( n＝9)。결론해방법쾌속간편，은정가고，전속성강。
Objective To establish a HPLC method for determining the hyPerin content in Rosa chinensis jacq. Methods The Agilent C18 column ( 100 mm × 4. 6 mm, 2. 7 μm ) was adoPted with the mobile of acetonitrile-0. 05% PhosPhoric acid ( 16 : 84 ); the detection wavelength was set at 360 nm with flow rate of 0. 5 mL/min. Results A good linearity relation was observed between the samPle size of hyPerin during the range of 0. 047 68-0. 476 80μg with the Peak area. The average recovery rate of hyPerin was 97. 22%, RSD=0. 89% ( n=9 ) . Conclusion This method is raPid, convenient, stable, reliable and strongly sPecific for the dermination of hyPerin in Rosa chinensis jacq.
